10/ N only 9 inpts, but a detailed early look at viral burdens from various sites, esp respiratory tract, over time, incl culture & the now-infamous subgenomic RNA, with paired serology (anti-spike IgG/M and neutralization). VL & culturability peak
RT @canchemistry: 「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようで…
@Kevin_McKernan @MRahnasto @realPseudonyymi @OssiTiihonen @lettipitko @AnuJaaskelainen @MichaelYeadon3 Could you please remind which Wölfel et al. paper you mean? Here, they actually used the Corman et al. protocol. https://t.co/jf7oJcqoXQ
RT @Kevin_McKernan: Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infe…
@MikaelNiku @OssiTiihonen @realPseudonyymi @lettipitko @AnuJaaskelainen @MichaelYeadon3 More uninformed excuses. See Wolfel et al. Drosten is a co-author. See Liotti et al. https://t.co/OiEsUOhMWz
@gallagher_grant Easy. Sars1 virus shedding was 1000 lower and after symptoms onset, so TETRIS worked and it might have favoured a less virulent variant spreading. None of that happens with Sars-CoV-2 because nasopharyngeal spreading seems to peak before s
@JoyJan15 the first paper that virus is non-infectious after 7.7 days is here: https://t.co/LNuJybA1dt
@Metabo_PhD Yes sorry I misunderstood your whole point and nit picked a single part of your thread. I agree about the fomite. Source though: 7.11*10^8 per 1 swab/ml on day 4 peak https://t.co/464n4vnoXH
@bslagter @harold_bos @DennisZeilstra @MarionKoopmans @hugodejonge Dit was er een van: https://t.co/yeOS1kc9x2 En deze ook: https://t.co/uKJj91HC3w
@JShendure Anyone compared N Vs ORF1a qPCR Ct? Subgenomic/genomic https://t.co/6sOdlUaoEj https://t.co/HJZ9v87Gpd https://t.co/pPCSeygViA https://t.co/khYH2vnGjr Kim https://t.co/CKk81iUkUq Tavares https://t.co/7fUTg5ZExj Alexandersen https://t.co/9MqiCTE
@LookMau Hier een uiteenzetting door Duitse wetenschappers: https://t.co/TsQfMB2Ul8 en hier die van Koreaanse: https://t.co/TFco2aGY2z
"We believe it is more likely due to coronavirus membrane-associated RNAs being partly, albeit not fully, protected from host and environmental degradation.Partial degradation,shorter average read lengths,may result in some shorter amplicon targets being p
RT @Kevin_McKernan: @MichaelYeadon3 Have look at the complexity of subgenomic RNA sgRNA https://t.co/6sOdlUaoEj https://t.co/HJZ9v87Gpd ht…
"The insertion of a polybasic cleavage site in the S1–S2 region in SARS-CoV has previously been shown to lead to a moderate, but discernible, gain-of-fusion activity that might result in increased viral entry in tissues with a low density of ACE2 expressio
@MichaelYeadon3 Have look at the complexity of subgenomic RNA sgRNA https://t.co/6sOdlUaoEj https://t.co/HJZ9v87Gpd https://t.co/pPCSeygViA https://t.co/khYH2vnGjr Kim https://t.co/CKk81iUkUq Tavares https://t.co/7fUTg5ZExj Alexandersen https://t.co/9MqiC
RT @Kevin_McKernan: @FransPietersma That will lead you through these papers. sgRNA https://t.co/6sOdlUaoEj https://t.co/HJZ9v87Gpd https:/…
RT @Kevin_McKernan: @FransPietersma That will lead you through these papers. sgRNA https://t.co/6sOdlUaoEj https://t.co/HJZ9v87Gpd https:/…
@FransPietersma That will lead you through these papers. sgRNA https://t.co/6sOdlUaoEj https://t.co/HJZ9v87Gpd https://t.co/pPCSeygViA https://t.co/khYH2vnGjr Kim https://t.co/CKk81iUkUq Tavares https://t.co/7fUTg5ZExj Alexandersen https://t.co/9MqiCTETFO
@Judgement84 @MelissaMilham @DollarBayDj @justmyo80178031 @ImSpeaking13 “In general, the concentrations of viral RNA were very high in initial samples. In all patients except one, the concentration of viral RNA in throat swabs seemed to be already on the d
RT @canchemistry: 「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようで…
RT @canchemistry: 「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようで…
RT @canchemistry: 「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようで…
RT @canchemistry: 「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようで…
@cggbamford Apologies, we've several separate exchanges going. https://t.co/T8DJLSZqYA https://t.co/fnqZPMPCtq
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @canchemistry: 症状の一つとして「嗅覚・味覚障害」があります。 ①COVID患者9人中4人に味覚・嗅覚障害が起こり、「一般的な風邪も強く、より長く続く」報告です。 また、発症後の最初の5日間にsgRNAが検出されたことから、軽症・前駆症状の段階で強い感染力を…
RT @canchemistry: 「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようで…
RT @canchemistry: 「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようで…
「sgRNAは、ウイルスが活発に増殖していたことを示す」については、以下も参考下さい(Nature 2020, 581, 465.) 一部には「PCR検査は、偶然に粘膜に付着した非感染性ウイルスを増幅しているだけ」という珍妙な意見があるようですが、sgRNAの存在によって完全に否定されます。 https://t.co/bdN2PTFhbJ https://t.co/9bG07b8t3A
Mayo de 2020 Valoración virológica de pacientes hospitalizados con COVID-2019 https://t.co/euGNykJh8X
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
#COVID2019 is contagious like a cold and letal as flu https://t.co/YVhFBG3Zj8 Virological assessment of hospitalized patients with COVID-2019 https://t.co/VP7492sIfM
RT @Bobby_Network: This! https://t.co/IzPYiloxBW
This!
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
@Nieuwsuur @hommel_b Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation. Wolfel et al explain how to do that here... and Drosten is on the paper. This would have been a good time to pull
Wirusologiczna ocena pacjentów hospitalizowanych z COVID-2019 Virological assessment of hospitalized patients with COVID-2019 https://t.co/spEyEgRumM
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
RT @Kevin_McKernan: Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation.…
Some have said that no qPCR can discern infect/non-infect RNA and you are just constructing an impossible expectation. Wolfel et al explain how to do that here... and Drosten is on the paper. This would have been a good time to pull the hasty assay. htt
RT @Kevin_McKernan: WTS, Im still impressed with his work in Wolfel et al and now have a better understanding of why they aimed to improve…
RT @Bobby_Network: next review report @c_drosten ?https://t.co/7UpEWHNWAu https://t.co/CVu5R1Ia1Q
RT @Bobby_Network: next review report @c_drosten ?https://t.co/7UpEWHNWAu
next review report @c_drosten ?https://t.co/7UpEWHNWAu
@NickHudsonCT @maestro_rayo @ElonBachman @imetatronink @federicolois Maybe suppression worked with SARS because the virus load pattern is completely different than in SARS-CoV-2: It peaks after 7-10 days of symptoms, is 1000 times lower and successful isol
@AndreasAtETH @marcelsalathe @C_Althaus @ASlavitt @MackayIM @SwissScience_TF Fig. 2 should go into the right direction https://t.co/psiO2JNs32
RT @Kevin_McKernan: WTS, Im still impressed with his work in Wolfel et al and now have a better understanding of why they aimed to improve…
WTS, Im still impressed with his work in Wolfel et al and now have a better understanding of why they aimed to improve the PCR process with sgRNA PCR in that paper. That would have been a great paper to point out the shortcomings of the prior primers. http
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
Viral load infectiousness https://t.co/iCHuQ3YDqe
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
RT @Kevin_McKernan: And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases th…
And he knows it. He's the communicating author on the Wolfel paper. Which is a very good paper and clearly showcases the long qPCR positivity and lack of infectiousness. https://t.co/OiEsUOhMWz
RT @chungk1031: There is a report that demonstrates active virus via cell culture cannot be isolated from the respiratory system after abou…
@DrEricDing Next paper for Dr Bing Bat: https://t.co/ikO1wlTIxt
@DrPieterPeach @Don_Milton Wolfel has some work on this front. https://t.co/OiEsUOhMWz
Only one study referenced in the Lancet Microbe SR&MA attempted to culture sputum from a cohort of 16 patients with mild disease: 5/n https://t.co/mRGKWGCBui
@Unvernunft2 @Karl_Lauterbach @volxfahradler @c_drosten Bei <1 Mio. RNA-Kopien/ml sind ca. 10% infektiös (erfolgreiche Virusanzucht). Trotzdem gilt: Weiterhin #AHAAL bei negativem Antigentest! https://t.co/XVbTBKsaBG https://t.co/tCX900rszj https://
One question: why aren't we just PCR testing for replicative (subgenomic) virus RNA instead? The answer is that these replicative RNA are at very low quantities, difficult to detect compared to straight up virus genomic RNA. https://t.co/oZtl6ywgA2 https:
RT @Kevin_McKernan: Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infe…
RT @Kevin_McKernan: Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infe…
RT @Kevin_McKernan: Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infe…
Virological assessment of hospitalized patients with COVID-2019 https://t.co/YYNjipJkkC
RT @Kevin_McKernan: Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infe…
RT @Kevin_McKernan: Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infe…
RT @Kevin_McKernan: Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infe…
Before we do the deep dive on Subgenomic RNA, Have a look at how Wolfel et al used this to track Infectious vs non-infectious (Live-Dead) SARs-CoV-2. Wolfel https://t.co/g0I1bRcZiy
@MichaelYeadon3 @ClareCraigPath @carlheneghan Asymptomatic non infectious dormancy is a bitch. But please line up for your daily lashings from zero FPR team humility. https://t.co/g0I1bRcZiy https://t.co/jFODUATxaF
Sars 8 https://t.co/wAV4lNVMVk
@Wheelzter @dean18892 @warren00761692 @CMO_England Oh FFS. Have two. There's more, but I can't be arsed looking at the moment, so knock yourself out. https://t.co/NAPd2wOGtD https://t.co/G4mp3kkNNu
@RebbeTakes @MaartenvSmeden Not true. SARS-CoV mostly spread in the later phase. https://t.co/TKIqQkowhm SARS-CoV-2 has a furin site that allows for much greater early replication in the nasal passages. https://t.co/sBbvw7bXcr https://t.co/au20BYYVtM
@hughosmond @alanmcn1 @whippletom @JuliaHB1 @cjsnowdon Ah, here it is... https://t.co/hf2mvXCLla And I take it you've seen this thread, right? https://t.co/bDN5flBp8R
@aperfectstrange @realmobius01 @ModernCortes @sentientist Correct. That tendency is related to the furin site. Note there are absolutely no other known examples of a furin site in this class of coronaviruses. See this discussion: https://t.co/Ea3GQc3KY
@takija2002 @RapidtestsDE Ja, aber die Korrelation VL/Viruskultivierbarkeit wurde ja in einigen Studien gezeigt (z.B. https://t.co/t0LxgCuBLG). Und da Ag-Tests eine niedrigere Gesamtsensitivität/höheres LOD haben, ist es nicht überraschend, dass sie besser
@CheeHongWong It is Corman who chose this scheme I guess he had a good reason (not using N) https://t.co/1p8m5PQLQg
#COVID2019 is contagious like a cold and letal as flu https://t.co/5fgF4pVzqI Virological assessment of hospitalized patients with COVID-2019 https://t.co/QwVwpRuSgh
RT @kenmomd: ウイルスが培養可能か Duration of Isolation and Precautions for Adults with COVID-19 https://t.co/Bcni5152p3 Virological assessment of ho…
RT @uchida_kawasaki: Virological assessment of hospitalized patients with COVID-2019 | Nature https://t.co/LFDdwNcFZh COVID-2019で入院した患者のウイ…
RT @mebaruhirame: コロナ 存在部位8 メモ 比較的症状の軽い9人。 Sputum痰が一番多い。 Stool便も多い。 (初期は検査データなし) Swab喉の綿棒少ないが発症後 一週間は高い。 四日目ピーク10億個/綿棒 Pharyngeal virus sh…
RT @mebaruhirame: コロナ 存在部位8 メモ 比較的症状の軽い9人。 Sputum痰が一番多い。 Stool便も多い。 (初期は検査データなし) Swab喉の綿棒少ないが発症後 一週間は高い。 四日目ピーク10億個/綿棒 Pharyngeal virus sh…
RT @mebaruhirame: コロナ 存在部位8 メモ 比較的症状の軽い9人。 Sputum痰が一番多い。 Stool便も多い。 (初期は検査データなし) Swab喉の綿棒少ないが発症後 一週間は高い。 四日目ピーク10億個/綿棒 Pharyngeal virus sh…
RT @mebaruhirame: コロナ 存在部位8 メモ 比較的症状の軽い9人。 Sputum痰が一番多い。 Stool便も多い。 (初期は検査データなし) Swab喉の綿棒少ないが発症後 一週間は高い。 四日目ピーク10億個/綿棒 Pharyngeal virus sh…
@Jensi48940329 @matzenordwind @Clown_welt @OlafGersemann @welt Viel Spaß beim Lesen. https://t.co/ULqedB2pT9
コロナ 存在部位8 メモ 比較的症状の軽い9人。 Sputum痰が一番多い。 Stool便も多い。 (初期は検査データなし) Swab喉の綿棒少ないが発症後 一週間は高い。 四日目ピーク10億個/綿棒 Pharyngeal virus shedding a peak at 7.11 × 10 8RNA copies per throat swab . 原本 https://t.co/GrVxZu78Qj https://t.co/gdVuCBrl5M
RT @vmcorman: Our latest contribution #ChariteVirology @ChariteBerlin @MarcelAMller @J_Schneider__ @bundeswehrInfo @c_drosten https://t.co…