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Mendeley readers
Attention Score in Context
| Title |
Crude subcellular fractionation of cultured mammalian cell lines
|
|---|---|
| Published in |
BMC Research Notes, December 2009
|
| DOI | 10.1186/1756-0500-2-243 |
| Pubmed ID | |
| Authors |
Paul Holden, William A Horton |
| Abstract |
The expression and study of recombinant proteins in mammalian culture systems can be complicated during the cell lysis procedure by contaminating proteins from cellular compartments distinct from those within which the protein of interest resides and also by solubility issues that may arise from the use of a single lysis buffer. Partial subcellular fractionation using buffers of increasing stringency, rather than whole cell lysis is one way in which to avoid or reduce this contamination and ensure complete recovery of the target protein. Currently published protocols involve time consuming centrifugation steps which may require expensive equipment and commercially available kits can be prohibitively expensive when handling large or multiple samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 534 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 8 | 1% |
| Germany | 5 | <1% |
| United Kingdom | 4 | <1% |
| South Africa | 2 | <1% |
| Italy | 1 | <1% |
| Brazil | 1 | <1% |
| India | 1 | <1% |
| Portugal | 1 | <1% |
| Austria | 1 | <1% |
| Other | 3 | <1% |
| Unknown | 507 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 149 | 28% |
| Researcher | 126 | 24% |
| Student > Master | 52 | 10% |
| Student > Bachelor | 44 | 8% |
| Student > Doctoral Student | 33 | 6% |
| Other | 67 | 13% |
| Unknown | 63 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 201 | 38% |
| Biochemistry, Genetics and Molecular Biology | 156 | 29% |
| Medicine and Dentistry | 26 | 5% |
| Chemistry | 23 | 4% |
| Immunology and Microbiology | 19 | 4% |
| Other | 26 | 5% |
| Unknown | 83 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2022.
All research outputs
#7,356,550
of 25,374,917 outputs
Outputs from BMC Research Notes
#1,103
of 4,513 outputs
Outputs of similar age
#43,377
of 175,876 outputs
Outputs of similar age from BMC Research Notes
#3
of 14 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 4,513 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 175,876 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.