Title |
Genetic transformation of lignin degrading fungi facilitated by Agrobacterium tumefaciens
|
---|---|
Published in |
BMC Biotechnology, September 2010
|
DOI | 10.1186/1472-6750-10-67 |
Pubmed ID | |
Authors |
Krishna K Sharma, Ramesh C Kuhad |
Abstract |
White-rot fungi are primarily the major degraders of lignin, a major obstacle for commercial exploitation of plant byproducts to produce bioethanol and other industrially important products. However, to improve their efficacy for lignin degradation, it has become necessary to genetically modify these organisms using appropriate vectors. Agrobacterium tumefaciens, a soil phytopathogenic bacterium, generally transforms plants by delivering a portion of the resident Ti- plasmid, the T-DNA (transfer DNA). The trans-Kingdom gene transfer is initiated by the activity of Ti-plasmid encoded vir (virulence) genes in response to low-molecular-mass phenolic compounds such as acetosyringone. A. tumefaciens played a major role in plant genetic engineering and basic research in molecular biology, accounting for nearly 80% of the transgenic plants produced so far. Initially, it was believed that only dicotyledons, gymnosperms and a few monocotyledonous species could be transformed by this bacterium; but recent reports have totally changed this scenario by demonstrating that many 'recalcitrant' species not included in its natural host range can also be transformed, especially filamentous fungi. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
United Kingdom | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
Switzerland | 1 | <1% |
Chile | 1 | <1% |
Italy | 1 | <1% |
Sweden | 1 | <1% |
South Africa | 1 | <1% |
Japan | 1 | <1% |
Greece | 1 | <1% |
United States | 1 | <1% |
Other | 0 | 0% |
Unknown | 128 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 29 | 21% |
Student > Ph. D. Student | 28 | 20% |
Student > Bachelor | 13 | 9% |
Student > Master | 12 | 9% |
Student > Postgraduate | 9 | 7% |
Other | 27 | 20% |
Unknown | 19 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 73 | 53% |
Biochemistry, Genetics and Molecular Biology | 18 | 13% |
Environmental Science | 4 | 3% |
Engineering | 4 | 3% |
Chemical Engineering | 2 | 1% |
Other | 11 | 8% |
Unknown | 25 | 18% |