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Antisense peptide nucleic acid-mediated knockdown of the p75 neurotrophin receptor delays motor neuron disease in mutant SOD1 transgenic mice

Overview of attention for article published in Journal of Neurochemistry, November 2003
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (76th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (62nd percentile)

Mentioned by

patent
2 patents

Citations

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65 Dimensions

Readers on

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40 Mendeley
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Title
Antisense peptide nucleic acid-mediated knockdown of the p75 neurotrophin receptor delays motor neuron disease in mutant SOD1 transgenic mice
Published in
Journal of Neurochemistry, November 2003
DOI 10.1046/j.1471-4159.2003.02053.x
Pubmed ID
Authors

Bradley J. Turner, Irwin K. Cheah, Katherine J. Macfarlane, Elizabeth C. Lopes, Steven Petratos, Steven J. Langford, Surindar S. Cheema

Abstract

Re-expression of the death-signalling p75 neurotrophin receptor (p75NTR) is associated with injury and neurodegeneration in the adult nervous system. The induction of p75NTR expression in mature degenerating spinal motor neurons of humans and transgenic mice with amyotrophic lateral sclerosis (ALS) suggests a role of p75NTR in the progression of motor neuron disease (MND). In this study, we designed, synthesized and evaluated novel antisense peptide nucleic acid (PNA) constructs targeting p75NTR as a potential gene knockdown therapeutic strategy for ALS. An 11-mer antisense PNA directed at the initiation codon, but not downstream gene sequences, dose-dependently inhibited p75NTR expression and death-signalling by nerve growth factor (NGF) in Schwann cell cultures. Antisense phosphorothioate oligonucleotide (PS-ODN) sequences used for comparison failed to confer such inhibitory activity. Systemic intraperitoneal administration of this antisense PNA to mutant superoxide dismutase 1 (SOD1G93A) transgenic mice significantly delayed locomotor impairment and mortality compared with mice injected with nonsense or scrambled PNA sequences. Reductions in p75NTR expression and subsequent caspase-3 activation in spinal cords were consistent with increased survival in antisense PNA-treated mice. The uptake of fluorescent-labelled antisense PNA in the nervous system of transgenic mice was also confirmed. This study suggests that p75NTR may be a promising antisense target in the treatment of ALS.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 2 5%
United States 1 3%
Unknown 37 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 28%
Student > Bachelor 11 28%
Researcher 7 18%
Professor 3 8%
Student > Master 2 5%
Other 6 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 33%
Medicine and Dentistry 10 25%
Biochemistry, Genetics and Molecular Biology 7 18%
Neuroscience 6 15%
Chemistry 3 8%
Other 1 3%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 August 2012.
All research outputs
#1,843,101
of 11,275,925 outputs
Outputs from Journal of Neurochemistry
#618
of 5,515 outputs
Outputs of similar age
#64,481
of 277,857 outputs
Outputs of similar age from Journal of Neurochemistry
#29
of 77 outputs
Altmetric has tracked 11,275,925 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,515 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,857 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.