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Validation of interphase fluorescence in situ hybridization (iFISH) for multiple myeloma using CD138 positive cells

Overview of attention for article published in Revista Brasileira de Hematologia e Hemoterapia, April 2016
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Title
Validation of interphase fluorescence in situ hybridization (iFISH) for multiple myeloma using CD138 positive cells
Published in
Revista Brasileira de Hematologia e Hemoterapia, April 2016
DOI 10.1016/j.bjhh.2016.01.005
Pubmed ID
Authors

Renata Kiyomi Kishimoto, Sarah Lee Vaughan Vulcani de Freitas, Cristina Alonso Ratis, Daniela Borri, Roberta Sitnik, Elvira Deolinda Rodrigues Pereira Velloso

Abstract

Multiple myeloma is a plasma cell neoplasm with acquired genetic abnormalities of clinical and prognostic importance. Multiple myeloma differs from other hematologic malignancies due to a high fraction of low proliferating malignant plasma cells and the paucity of plasma cells in bone marrow aspiration samples, making cytogenetic analysis a challenge. An abnormal karyotype is found in only one-third of patients with multiple myeloma and interphase fluorescence in situ hybridization is the most useful test for studying the chromosomal abnormalities present in almost 90% of cases. However, it is necessary to study the genetic abnormalities in plasma cells after their identification or selection by morphology, immunophenotyping or sorting. Other challenges are the selection of the most informative FISH panel and determining cut-off levels for FISH probes. This study reports the validation of interphase fluorescence in situ hybridization using CD138 positive cells, according to proposed guidelines published by the European Myeloma Network (EMN) in 2012. Bone marrow samples from patients with multiple myeloma were used to standardize a panel of five probes [1q amplification, 13q14 deletion, 17p deletion, t(4;14), and t(14;16)] in CD138(+) cells purified by magnetic cell sorting. This test was validated with a low turnaround time and good reproducibility. Five of six samples showed genetic abnormalities. Monosomy/deletion 13 plus t(4;14) were found in two cases. This technique together with magnetic cell sorting is effective and can be used in the routine laboratory practice. In addition, magnetic cell sorting provides a pure plasma cell population that allows other molecular and genomic studies.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Other 5 17%
Student > Bachelor 5 17%
Professor > Associate Professor 4 13%
Student > Master 4 13%
Student > Ph. D. Student 3 10%
Other 6 20%
Unknown 3 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 30%
Medicine and Dentistry 9 30%
Agricultural and Biological Sciences 5 17%
Engineering 2 7%
Immunology and Microbiology 2 7%
Other 2 7%
Unknown 1 3%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 May 2016.
All research outputs
#9,964,998
of 12,447,688 outputs
Outputs from Revista Brasileira de Hematologia e Hemoterapia
#127
of 222 outputs
Outputs of similar age
#186,345
of 267,529 outputs
Outputs of similar age from Revista Brasileira de Hematologia e Hemoterapia
#1
of 2 outputs
Altmetric has tracked 12,447,688 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 222 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,529 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them