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Mendeley readers
Attention Score in Context
| Title |
Differentiation of human mesenchymal stem cell spheroids under microgravity conditions
|
|---|---|
| Published in |
Cell Regeneration, June 2012
|
| DOI | 10.1186/2045-9769-1-2 |
| Pubmed ID | |
| Authors |
Wolfgang H Cerwinka, Starlette M Sharp, Barbara D Boyan, Haiyen E Zhau, Leland W K Chung, Clayton Yates |
| Abstract |
To develop and characterize a novel cell culture method for the generation of undifferentiated and differentiated human mesenchymal stem cell 3D structures, we utilized the RWV system with a gelatin-based scaffold. 3 × 10(6) cells generated homogeneous spheroids and maximum spheroid loading was accomplished after 3 days of culture. Spheroids cultured in undifferentiated spheroids of 3 and 10 days retained expression of CD44, without expression of differentiation markers. Spheroids cultured in adipogenic and osteogenic differentiation media exhibited oil red O staining and von Kossa staining, respectively. Further characterization of osteogenic lineage, showed that 10 day spheroids exhibited stronger calcification than any other experimental group corresponding with significant expression of vitamin D receptor, alkaline phosphatase, and ERp60 . In conclusion this study describes a novel RWV culture method that allowed efficacious engineering of undifferentiated human mesenchymal stem cell spheroids and rapid osteogenic differentiation. The use of gelatin scaffolds holds promise to design implantable stem cell tissue of various sizes and shapes for future regenerative treatment. |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 1% |
| Romania | 1 | 1% |
| Unknown | 67 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 17% |
| Student > Master | 12 | 17% |
| Researcher | 10 | 14% |
| Student > Doctoral Student | 8 | 12% |
| Student > Postgraduate | 7 | 10% |
| Other | 13 | 19% |
| Unknown | 7 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 23% |
| Biochemistry, Genetics and Molecular Biology | 15 | 22% |
| Medicine and Dentistry | 14 | 20% |
| Engineering | 8 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Other | 3 | 4% |
| Unknown | 11 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2012.
All research outputs
#5,422,967
of 25,374,647 outputs
Outputs from Cell Regeneration
#30
of 189 outputs
Outputs of similar age
#35,919
of 177,594 outputs
Outputs of similar age from Cell Regeneration
#1
of 4 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 189 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 177,594 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them