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Differentiation of human mesenchymal stem cell spheroids under microgravity conditions

Overview of attention for article published in Cell Regeneration, January 2012
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (82nd percentile)

Mentioned by

1 blog


20 Dimensions

Readers on

45 Mendeley
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Differentiation of human mesenchymal stem cell spheroids under microgravity conditions
Published in
Cell Regeneration, January 2012
DOI 10.1186/2045-9769-1-2
Pubmed ID

Wolfgang H Cerwinka, Starlette M Sharp, Barbara D Boyan, Haiyen E Zhau, Leland W K Chung, Clayton Yates


To develop and characterize a novel cell culture method for the generation of undifferentiated and differentiated human mesenchymal stem cell 3D structures, we utilized the RWV system with a gelatin-based scaffold. 3 × 10(6) cells generated homogeneous spheroids and maximum spheroid loading was accomplished after 3 days of culture. Spheroids cultured in undifferentiated spheroids of 3 and 10 days retained expression of CD44, without expression of differentiation markers. Spheroids cultured in adipogenic and osteogenic differentiation media exhibited oil red O staining and von Kossa staining, respectively. Further characterization of osteogenic lineage, showed that 10 day spheroids exhibited stronger calcification than any other experimental group corresponding with significant expression of vitamin D receptor, alkaline phosphatase, and ERp60 . In conclusion this study describes a novel RWV culture method that allowed efficacious engineering of undifferentiated human mesenchymal stem cell spheroids and rapid osteogenic differentiation. The use of gelatin scaffolds holds promise to design implantable stem cell tissue of various sizes and shapes for future regenerative treatment.

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Iran, Islamic Republic of 1 2%
Romania 1 2%
Unknown 43 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 11 24%
Student > Ph. D. Student 9 20%
Researcher 7 16%
Student > Doctoral Student 5 11%
Student > Postgraduate 4 9%
Other 7 16%
Unknown 2 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 36%
Biochemistry, Genetics and Molecular Biology 10 22%
Medicine and Dentistry 9 20%
Engineering 5 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 0 0%
Unknown 4 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2012.
All research outputs
of 12,246,070 outputs
Outputs from Cell Regeneration
of 38 outputs
Outputs of similar age
of 115,862 outputs
Outputs of similar age from Cell Regeneration
of 3 outputs
Altmetric has tracked 12,246,070 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 38 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 10.0. This one scored the same or higher as 26 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 115,862 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them