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Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

Overview of attention for article published in Revista da Sociedade Brasileira de Medicina Tropical, December 2015
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Title
Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil
Published in
Revista da Sociedade Brasileira de Medicina Tropical, December 2015
DOI 10.1590/0037-8682-0076-2015
Pubmed ID
Authors

Tauana de Sousa Ferreira, Thaís Tâmara Castro Minuzzi-Souza, Andrey José de Andrade, Thais Oliveira Coelho, Douglas de Almeida Rocha, Marcos Takashi Obara, Mariana Hecht, Nadjar Nitz, Rodrigo Gurgel-Gonçalves

Abstract

This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.

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Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 39%
Student > Bachelor 2 11%
Other 2 11%
Student > Ph. D. Student 1 6%
Professor 1 6%
Other 2 11%
Unknown 3 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 39%
Veterinary Science and Veterinary Medicine 3 17%
Immunology and Microbiology 2 11%
Environmental Science 1 6%
Medicine and Dentistry 1 6%
Other 0 0%
Unknown 4 22%