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Host-Bacteria Interactions

Overview of attention for book
Cover of 'Host-Bacteria Interactions'

Table of Contents

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    Book Overview
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    Chapter 1 Galleria mellonella as an Infection Model for Select Agents.
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    Chapter 2 Drosophila as a model for intestinal infections.
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    Chapter 3 Zebrafish embryos as a model to study bacterial virulence.
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    Chapter 4 Studying host-pathogen interaction events in living mice visualized in real time using biophotonic imaging.
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    Chapter 5 Intravital two-photon imaging to understand bacterial infections of the Mammalian host.
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    Chapter 6 Cre Reporter Assay for Translocation (CRAfT): A Tool for the Study of Protein Translocation into Host Cells.
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    Chapter 7 Detection of the interaction between host and bacterial proteins: eukaryotic nucleolin interacts with francisella elongation factor tu.
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    Chapter 8 Hijacking the host proteasome for the temporal degradation of bacterial effectors.
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    Chapter 9 Live cell imaging of phosphoinositide dynamics during legionella infection.
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    Chapter 10 Investigating interference with apoptosis induction by bacterial proteins.
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    Chapter 11 Bimolecular fluorescence complementation for imaging protein interactions in plant hosts of microbial pathogens.
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    Chapter 12 Investigating TLR Signaling Responses in Murine Dendritic Cells Upon Bacterial Infection.
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    Chapter 13 siRNA Screens Using Drosophila Cells to Identify Host Factors Required for Infection.
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    Chapter 14 Purification of Intracellular Bacteria: Isolation of Viable Brucella abortus from Host Cells.
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    Chapter 15 RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens.
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    Chapter 16 Taking the shortcut for high-throughput shotgun proteomic analysis of bacteria.
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    Chapter 17 Comparative Genomic Analysis at the PATRIC, A Bioinformatic Resource Center.
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    Chapter 18 A Markerless Deletion Method for Genetic Manipulation of Burkholderia cenocepacia and Other Multidrug-Resistant Gram-Negative Bacteria.
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    Chapter 19 Gene Inactivation in Coxiella burnetii.
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    Chapter 20 A Chemical Mutagenesis Approach to Identify Virulence Determinants in the Obligate Intracellular Pathogen Chlamydia trachomatis.
Attention for Chapter 15: RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens.
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Chapter title
RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens.
Chapter number 15
Book title
Host-Bacteria Interactions
Published in
Methods in molecular biology, July 2014
DOI 10.1007/978-1-4939-1261-2_15
Pubmed ID
25172286
Book ISBNs
978-1-4939-1260-5, 978-1-4939-1261-2
Authors

Julien Rougeot, Ania Zakrzewska, Zakia Kanwal, Hans J Jansen, Herman P Spaink, Annemarie H Meijer, Hans J. Jansen, Herman P. Spaink, Annemarie H. Meijer

Editors

Annette C. Vergunst, David O'Callaghan

Abstract

The zebrafish (Danio rerio) is increasingly used as a model for studying infectious diseases. This nonmammalian vertebrate host, which is transparent at the early life stages, is especially attractive for live imaging of interactions between pathogens and host cells. A number of useful fluorescent reporter lines have recently been developed and significant advances in RNA sequencing technology have been made, which now make it possible to apply the zebrafish model for investigating changes in transcriptional activity of specific immune cell types during the course of an infection process.Here we describe how to sequence RNA extracted from fluorescently labeled macrophages obtained by cell-sorting of 5-day-old zebrafish larvae of the transgenic Tg(mpeg1:Gal4-VP16);Tg(UAS-E1b:Kaede) line. This technique showed reproducible results and allowed to detect specific expression of macrophage markers in the mpeg1 positive cell population, whereas no markers specific for neutrophils or lymphoid cells were detected. This protocol has been also successfully extended to other immune cell types as well as cells infected by Mycobacterium marinum.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
United States 1 2%
South Africa 1 2%
Unknown 50 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 26%
Student > Master 9 17%
Researcher 5 9%
Student > Postgraduate 4 8%
Student > Bachelor 4 8%
Other 11 21%
Unknown 6 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 32%
Biochemistry, Genetics and Molecular Biology 15 28%
Immunology and Microbiology 5 9%
Chemistry 3 6%
Medicine and Dentistry 2 4%
Other 4 8%
Unknown 7 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 August 2014.
All research outputs
#20,235,415
of 22,761,738 outputs
Outputs from Methods in molecular biology
#9,864
of 13,088 outputs
Outputs of similar age
#192,681
of 228,768 outputs
Outputs of similar age from Methods in molecular biology
#38
of 92 outputs
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So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 92 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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