Chapter title |
Investigating TLR Signaling Responses in Murine Dendritic Cells Upon Bacterial Infection.
|
---|---|
Chapter number | 12 |
Book title |
Host-Bacteria Interactions
|
Published in |
Methods in molecular biology, July 2014
|
DOI | 10.1007/978-1-4939-1261-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1260-5, 978-1-4939-1261-2
|
Authors |
Suzana Pinto Salcedo, Lena Alexopoulou |
Editors |
Annette C. Vergunst, David O'Callaghan |
Abstract |
Innate immune recognition of microbial components is critical for the onset of an appropriate immune response against invading pathogens, in particular by dendritic cells. Toll-like receptors (TLRs) are key in the detection of a variety of microbial stimuli.Here we focus on the methodology used to evaluate the role of TLRs in the process of dendritic cell response to bacterial intracellular infections, using bone marrow-derived dendritic cells (BMDCs) as a model system. This protocol describes how to access the level of activation of BMDCs using standard immunology and biochemistry approaches along with examination of infected cells by immunofluorescence microscopy. |
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