Chapter title |
Detection of the interaction between host and bacterial proteins: eukaryotic nucleolin interacts with francisella elongation factor tu.
|
---|---|
Chapter number | 7 |
Book title |
Host-Bacteria Interactions
|
Published in |
Methods in molecular biology, July 2014
|
DOI | 10.1007/978-1-4939-1261-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1260-5, 978-1-4939-1261-2
|
Authors |
Barel M, Charbit A, Monique Barel, Alain Charbit, Barel, Monique, Charbit, Alain |
Editors |
Annette C. Vergunst, David O'Callaghan |
Abstract |
Dissecting the interaction between bacterial and host proteins is fundamental in understanding pathogenesis. It is also very helpful for exploring new therapeutic approaches, either preventive or curative. Here, we describe different techniques, which allowed us to detect new molecules involved in the binding and infection of the bacterium Francisella tularensis, on human cells. This facultative intracellular pathogen is the causative agent of tularemia and is considered as a bio-threatening agent. The privileged host cells are monocytes and macrophages. We used both "in vitro" and "in vivo" experiments to explore the modulation of F. tularensis infection and thereafter determine a bacterial ligand and its host receptor molecule. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 57% |
Researcher | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 43% |
Immunology and Microbiology | 3 | 43% |
Unknown | 1 | 14% |