↓ Skip to main content

Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis

Overview of attention for article published in Brazilian Journal of Infectious Diseases, May 2016
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
44 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis
Published in
Brazilian Journal of Infectious Diseases, May 2016
DOI 10.1016/j.bjid.2016.01.006
Pubmed ID
Authors

Reena Raveendran, Chand Wattal

Abstract

The diagnosis of extrapulmonary tuberculosis (EPTB) is still a challenge because of its pauci-bacillary nature. The aim of the study was to evaluate the role of a multiplex PCR assay in the diagnosis of extrapulmonary tuberculosis and to compare the efficiency of two targets, IS6110 and MPB64 to detect Mycobacterium tuberculosis. 150 extrapulmonary samples (61 pus/aspirate, 46 tissue, 32 body fluids, and 11 urine) from clinically suspected cases of tuberculosis were included in the study. All the samples were subjected to direct fluorescent microscopy, TB culture (BacT/ALERT 3D, biomerieux, Durham, North Carolina, USA) and a Multiplexed Tandem PCR targeting two mycobacterial DNA sequences, IS6110 and MPB64. Master-Mix reagents and primers were prepared by AusDiagnostics Pvt. Ltd (Alexandria, New South Wales, Australia). The performance of the assay was assessed using a composite gold standard (CGS), which included clinical characteristics, microbiology smear as well as culture, histopathology, cytology, radiology, and response to antitubercular therapy. 20.3%, 23.6%, and 45.3% of specimens were positive by smear, culture, and PCR, respectively. The sensitivity and specificity of the multiplex PCR was 91.9% and 88.4%, respectively, using the CGS. Positive and negative predictive values of the PCR were estimated as 85.1% and 93.8%, respectively. Higher positivity was observed with target IS6110 (44.6%) as compared to target MPB64 (18.9%). The sensitivities of IS6110 and MPB64 individual targets were 90.3% and 64.5%, respectively, and specificities were 88.4% and 97.7%, respectively. PCR can play an important role in rapid and accurate diagnosis of extrapulmonary tuberculosis. IS6110 alone is an effective target in our part of the country.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 43 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 18%
Student > Postgraduate 7 16%
Student > Master 7 16%
Student > Bachelor 6 14%
Student > Ph. D. Student 3 7%
Other 5 11%
Unknown 8 18%
Readers by discipline Count As %
Medicine and Dentistry 17 39%
Biochemistry, Genetics and Molecular Biology 8 18%
Immunology and Microbiology 5 11%
Nursing and Health Professions 3 7%
Agricultural and Biological Sciences 2 5%
Other 0 0%
Unknown 9 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2016.
All research outputs
#8,450,986
of 13,475,838 outputs
Outputs from Brazilian Journal of Infectious Diseases
#163
of 369 outputs
Outputs of similar age
#145,331
of 267,704 outputs
Outputs of similar age from Brazilian Journal of Infectious Diseases
#2
of 3 outputs
Altmetric has tracked 13,475,838 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 369 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,704 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.