Title |
Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
|
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Published in |
AMB Express, July 2012
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DOI | 10.1186/2191-0855-2-37 |
Pubmed ID | |
Authors |
Hironobu Morisaka, Kazuma Matsui, Yohei Tatsukami, Kouichi Kuroda, Hideo Miyake, Yutaka Tamaru, Mitsuyoshi Ueda |
Abstract |
We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901-902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 3% |
Unknown | 31 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 25% |
Researcher | 4 | 13% |
Professor > Associate Professor | 3 | 9% |
Student > Bachelor | 2 | 6% |
Student > Master | 2 | 6% |
Other | 4 | 13% |
Unknown | 9 | 28% |
Readers by discipline | Count | As % |
---|---|---|
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Biochemistry, Genetics and Molecular Biology | 5 | 16% |
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Chemical Engineering | 1 | 3% |
Earth and Planetary Sciences | 1 | 3% |
Other | 1 | 3% |
Unknown | 9 | 28% |