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The Mitotic Spindle

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Attention for Chapter 18: The Mitotic Spindle
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Chapter title
The Mitotic Spindle
Chapter number 18
Book title
The Mitotic Spindle
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3542-0_18
Pubmed ID
Book ISBNs
978-1-4939-3540-6, 978-1-4939-3542-0
Authors

Al-Obaidi, Naowras, Kastl, Johanna, Mayer, Thomas U, Naowras Al-Obaidi, Johanna Kastl, Thomas U. Mayer

Editors

Paul Chang, Ryoma Ohi

Abstract

Mitotic motor proteins of the kinesin superfamily are critical for the faithful segregation of chromosomes and the formation of the two daughter cells during meiotic and mitotic M-phase. Of the 45 human kinesins, roughly a dozen are involved in the assembly of the bipolar spindle, alignment of chromosomes at the spindle equator, chromosome segregation, and cytokinesis. The functions of kinesins in these processes are highly diverse and include the transport of cargo molecules, sliding and bundling of microtubules, and regulation of microtubule dynamics. In light of this multitude of diverse functions and the complex functional interplay of different kinesins during M-phase, it is not surprising that one of the greatest challenges in cell biology is the functional dissection of individual motor proteins. Reversible and fast acting small molecules are powerful tools to accomplish this challenge. However, the validity of conclusions drawn from small molecule studies strictly depends on compound specificity. In this chapter, we present methods for the identification of small molecule inhibitors of a motor protein of interest. In particular, we focus on a protein-based large throughput screen to identify inhibitors of the ATPase activity of kinesins. Furthermore, we provide protocols and guidelines for secondary screens to validate hits and select for specific inhibitors.

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Mendeley readers

Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Researcher 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2016.
All research outputs
#20,328,845
of 22,873,031 outputs
Outputs from Methods in molecular biology
#9,915
of 13,130 outputs
Outputs of similar age
#330,715
of 393,675 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
Altmetric has tracked 22,873,031 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,130 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.