Title |
Peptide sequences from the first Castoroides ohioensis skull and the utility of old museum collections for palaeoproteomics
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Published in |
Proceedings of the Royal Society B: Biological Sciences, June 2016
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DOI | 10.1098/rspb.2016.0593 |
Pubmed ID | |
Authors |
Timothy P. Cleland, Elena R. Schroeter, Robert S. Feranec, Deepak Vashishth |
Abstract |
Vertebrate fossils have been collected for hundreds of years and are stored in museum collections around the world. These remains provide a readily available resource to search for preserved proteins; however, the vast majority of palaeoproteomic studies have focused on relatively recently collected bones with a well-known handling history. Here, we characterize proteins from the nasal turbinates of the first Castoroides ohioensis skull ever discovered. Collected in 1845, this is the oldest museum-curated specimen characterized using palaeoproteomic tools. Our mass spectrometry analysis detected many collagen I peptides, a peptide from haemoglobin beta, and in vivo and diagenetic post-translational modifications. Additionally, the identified collagen I sequences provide enough resolution to place C. ohioensis within Rodentia. This study illustrates the utility of archived museum specimens for both the recovery of preserved proteins and phylogenetic analyses. |
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Geographical breakdown
Country | Count | As % |
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United States | 10 | 37% |
Canada | 3 | 11% |
Japan | 1 | 4% |
Germany | 1 | 4% |
Syrian Arab Republic | 1 | 4% |
Denmark | 1 | 4% |
United Kingdom | 1 | 4% |
Unknown | 9 | 33% |
Demographic breakdown
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Members of the public | 19 | 70% |
Scientists | 8 | 30% |
Mendeley readers
Geographical breakdown
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Unknown | 42 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 11 | 26% |
Researcher | 7 | 17% |
Student > Bachelor | 6 | 14% |
Other | 3 | 7% |
Student > Master | 3 | 7% |
Other | 4 | 10% |
Unknown | 8 | 19% |
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Chemistry | 8 | 19% |
Agricultural and Biological Sciences | 5 | 12% |
Earth and Planetary Sciences | 4 | 10% |
Unspecified | 1 | 2% |
Other | 4 | 10% |
Unknown | 11 | 26% |