Chapter title |
Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS
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Chapter number | 36 |
Book title |
Mitochondrial Medicine
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2257-4_36 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2256-7, 978-1-4939-2257-4
|
Authors |
Susan S. Bird, Irina G. Stavrovskaya, Rose M. Gathungu, Fateme Tousi, Bruce S. Kristal |
Abstract |
Untargeted lipidomics profiling by liquid chromatography-mass spectrometry (LC-MS) allows researchers to observe the occurrences of lipids in a biological sample without showing intentional bias to any specific class of lipids and allows retrospective reanalysis of data collected. Typically, and in the specific method described, a general extraction method followed by LC separation is used to achieve nonspecific class coverage of the lipidome prior to high-resolution accurate mass (HRAM) MS detection. Here we describe a workflow including the isolation of mitochondria from liver tissue, followed by mitochondrial lipid extraction and the LC-MS conditions used for data acquisition. We also highlight how, in this method, all-ion fragmentation can be used to identify species of lower abundances, often missed by data-dependent fragmentation techniques. Here we describe the isolation of mitochondria from liver tissue, followed by mitochondrial lipid extraction and the LC-MS conditions used for data acquisition. |
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Unknown | 1 | 100% |
Demographic breakdown
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