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Mitochondrial Medicine

Overview of attention for book
Cover of 'Mitochondrial Medicine'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Preparation of “Functional” Mitochondria: A Challenging Business
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    Chapter 2 Isolation and Quality Control of Functional Mitochondria
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    Chapter 3 Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid-Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells
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    Chapter 4 Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels
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    Chapter 5 Optical microwell arrays for large-scale studies of single mitochondria metabolic responses.
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    Chapter 6 Deep resequencing of mitochondrial DNA.
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    Chapter 7 Single-cell analysis of mitochondrial DNA.
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    Chapter 8 A high-throughput next-generation sequencing assay for the mitochondrial genome.
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    Chapter 9 Rapid mitochondrial DNA isolation method for direct sequencing.
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    Chapter 10 Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR-Based Method
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    Chapter 11 Mitochondria-targeted RNA import.
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    Chapter 12 Accurate Measurement of Circulating Mitochondrial DNA Content from Human Blood Samples Using Real-Time Quantitative PCR
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    Chapter 13 mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution
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    Chapter 14 Simultaneous Quantification of Mitochondrial ATP and ROS Production.
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    Chapter 15 Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine.
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    Chapter 16 Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid
  18. Altmetric Badge
    Chapter 17 Mitochondrial Medicine
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    Chapter 18 Novel Methods for Measuring the Mitochondrial Membrane Potential
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    Chapter 19 High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes
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    Chapter 20 In Vivo Assessment of Mitochondrial Oxygen Consumption
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    Chapter 21 Imaging mitochondrial hydrogen peroxide in living cells.
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    Chapter 22 Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production.
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    Chapter 23 Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells
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    Chapter 24 Mitochondrial Coenzyme Q10 Determination via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry
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    Chapter 25 Assessing the Bioenergetic Profile of Human Pluripotent Stem Cells
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    Chapter 26 Integrative Methods for Studying Cardiac Energetics
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    Chapter 27 Computer-based prediction of mitochondria-targeting peptides.
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    Chapter 28 Prediction of mitochondrial protein function by comparative physiology and phylogenetic profiling.
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    Chapter 29 Assessment of posttranslational modification of mitochondrial proteins.
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    Chapter 30 Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway
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    Chapter 31 High-Resolution Melting Analysis for Identifying Sequence Variations in Nuclear Genes for Assembly Factors and Structural Subunits of Cytochrome C Oxidase
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    Chapter 32 Heterologous Inferential Analysis (HIA) as a Method to Understand the Role of Mitochondrial rRNA Mutations in Pathogenesis.
  34. Altmetric Badge
    Chapter 33 Analysis of Mitochondrial Dysfunction During Cell Death
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    Chapter 34 The Use of FLIM-FRET for the Detection of Mitochondria-Associated Protein Interactions
  36. Altmetric Badge
    Chapter 35 Mitochondrial Medicine
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    Chapter 36 Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS
  38. Altmetric Badge
    Chapter 37 Characterization of Mitochondrial Populations During Stem Cell Differentiation
  39. Altmetric Badge
    Chapter 38 An Ex Vivo Model for Studying Mitochondrial Trafficking in Neurons
Attention for Chapter 8: A high-throughput next-generation sequencing assay for the mitochondrial genome.
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Chapter title
A high-throughput next-generation sequencing assay for the mitochondrial genome.
Chapter number 8
Book title
Mitochondrial Medicine
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2257-4_8
Pubmed ID
Book ISBNs
978-1-4939-2256-7, 978-1-4939-2257-4
Authors

Shale Dames, Karen Eilbeck, Rong Mao

Editors

Volkmar Weissig, Marvin Edeas

Abstract

Next-generation sequencing (NGS) is an effective method for mitochondrial genome (mtDNA) sequencing and heteroplasmy detection. The following protocol describes an mtDNA enrichment method up to library preparation and sequencing on Illumina NGS platforms. A short command line alignment script is available for download via FTP.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 6%
Brazil 1 6%
Unknown 15 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 29%
Student > Ph. D. Student 5 29%
Professor > Associate Professor 2 12%
Student > Master 1 6%
Student > Doctoral Student 1 6%
Other 0 0%
Unknown 3 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 35%
Agricultural and Biological Sciences 4 24%
Environmental Science 1 6%
Medicine and Dentistry 1 6%
Engineering 1 6%
Other 0 0%
Unknown 4 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2015.
All research outputs
#20,251,805
of 22,780,967 outputs
Outputs from Methods in molecular biology
#9,885
of 13,094 outputs
Outputs of similar age
#295,679
of 352,963 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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So far Altmetric has tracked 13,094 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.