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Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods

Overview of attention for article published in Environmental Science & Pollution Research, June 2016
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Mentioned by

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3 tweeters

Citations

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6 Dimensions

Readers on

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15 Mendeley
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Title
Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods
Published in
Environmental Science & Pollution Research, June 2016
DOI 10.1007/s11356-016-7039-9
Pubmed ID
Authors

P. Gyawali, W. Ahmed, J. P. S. Sidhu, S. V. Nery, A. C. Clements, R. Traub, J. S. McCarthy, S. Llewellyn, P. Jagals, S. Toze, Gyawali, P, Ahmed, W, Sidhu, J P S, Nery, S V, Clements, A C, Traub, R, McCarthy, J S, Llewellyn, S, Jagals, P, Toze, S

Abstract

In this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) to differentiate between viable and non-viable Ancylostoma caninum ova. The newly developed method was validated using raw wastewater seeded with known numbers of A. caninum ova. Results of this study confirmed that PMA-qPCR has resulted in average of 88 % reduction (P < 0.05) in gene copy numbers for 50 % viable +50 % non-viable when compared with 100 % viable ova. A reduction of 100 % in gene copies was observed for 100 % non-viable ova when compared with 100 % viable ova. Similar reductions (79-80 %) in gene copies were observed for A. caninum ova-seeded raw wastewater samples (n = 18) collected from wastewater treatment plants (WWTPs) A and B. The newly developed PMA-qPCR method was applied to determine the viable ova of different helminths (A. caninum, A. duodenale, Necator americanus and Ascaris lumbricoides) in raw wastewater, human fecal and soil samples. None of the unseeded wastewater samples were positive for the above-mentioned helminths. N. americanus and A. lumbricoides ova were found in unseeded human fecal and soil samples. For the unseeded human fecal samples (1 g), an average gene copy concentration obtained from qPCR and PMA-qPCR was found to be similar (6.8 × 10(5) ± 6.4 × 10(5) and 6.3 × 10(5) ± 4.7 × 10(5)) indicating the presence of viable N. americanus ova. Among the 24 unseeded soil samples tested, only one was positive for A. lumbricoides. The mean gene copy concentration in the positively identified soil sample was 1.0 × 10(5) ± 1.5 × 10(4) (determined by qPCR) compared to 4.9 × 10(4) ± 3.7 × 10(3) (determined by PMA-qPCR). The newly developed PMA-qPCR methods were able to detect viable helminth ova from wastewater and soil samples and could be adapted for health risk assessment.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 40%
Student > Master 5 33%
Student > Ph. D. Student 1 7%
Other 1 7%
Professor > Associate Professor 1 7%
Other 1 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 27%
Environmental Science 3 20%
Unspecified 3 20%
Medicine and Dentistry 2 13%
Social Sciences 1 7%
Other 2 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 April 2017.
All research outputs
#4,929,600
of 9,725,367 outputs
Outputs from Environmental Science & Pollution Research
#702
of 2,746 outputs
Outputs of similar age
#116,332
of 252,285 outputs
Outputs of similar age from Environmental Science & Pollution Research
#30
of 141 outputs
Altmetric has tracked 9,725,367 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,746 research outputs from this source. They receive a mean Attention Score of 2.7. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,285 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 141 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.