↓ Skip to main content

Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death

Overview of attention for article published in BMC Research Notes, August 2012
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
1 X user

Citations

dimensions_citation
9 Dimensions

Readers on

mendeley
29 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Published in
BMC Research Notes, August 2012
DOI 10.1186/1756-0500-5-437
Pubmed ID
Authors

Kyle S Hubbard, Ian M Gut, Stephen M Scheeler, Megan E Lyman, Patrick M McNutt

Abstract

Simultaneous use of cell-permeant and impermeant fluorescent nuclear dyes is a common method to study cell viability and cell death progression. Although these assays are usually conducted as end-point studies, time-lapse imaging offers a powerful technique to distinguish temporal changes in cell viability at single-cell resolution. SYTO 13 and Hoechst 33342 are two commonly used cell-permeant nuclear dyes; however their suitability for live imaging has not been well characterized. We compare end-point assays with time-lapse imaging studies over a 6 h period to evaluate the compatibility of these two dyes with longitudinal imaging, using both control neurons and an apoptotic neuron model.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 1 3%
Unknown 28 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 45%
Student > Doctoral Student 3 10%
Student > Bachelor 2 7%
Student > Master 2 7%
Researcher 2 7%
Other 1 3%
Unknown 6 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 31%
Biochemistry, Genetics and Molecular Biology 7 24%
Chemistry 2 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Unspecified 1 3%
Other 2 7%
Unknown 6 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2012.
All research outputs
#15,248,503
of 22,673,450 outputs
Outputs from BMC Research Notes
#2,309
of 4,250 outputs
Outputs of similar age
#106,880
of 167,805 outputs
Outputs of similar age from BMC Research Notes
#56
of 102 outputs
Altmetric has tracked 22,673,450 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,250 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 167,805 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 102 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.