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Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death

Overview of attention for article published in BMC Research Notes, January 2012
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1 tweeter

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17 Mendeley
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Title
Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Published in
BMC Research Notes, January 2012
DOI 10.1186/1756-0500-5-437
Pubmed ID
Authors

Kyle S Hubbard, Ian M Gut, Stephen M Scheeler, Megan E Lyman, Patrick M McNutt

Abstract

Simultaneous use of cell-permeant and impermeant fluorescent nuclear dyes is a common method to study cell viability and cell death progression. Although these assays are usually conducted as end-point studies, time-lapse imaging offers a powerful technique to distinguish temporal changes in cell viability at single-cell resolution. SYTO 13 and Hoechst 33342 are two commonly used cell-permeant nuclear dyes; however their suitability for live imaging has not been well characterized. We compare end-point assays with time-lapse imaging studies over a 6 h period to evaluate the compatibility of these two dyes with longitudinal imaging, using both control neurons and an apoptotic neuron model.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 1 6%
Unknown 16 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 41%
Student > Doctoral Student 3 18%
Unspecified 3 18%
Researcher 1 6%
Student > Master 1 6%
Other 2 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 41%
Biochemistry, Genetics and Molecular Biology 5 29%
Unspecified 3 18%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Engineering 1 6%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2012.
All research outputs
#7,855,328
of 12,519,627 outputs
Outputs from BMC Research Notes
#1,420
of 2,804 outputs
Outputs of similar age
#68,152
of 121,783 outputs
Outputs of similar age from BMC Research Notes
#6
of 17 outputs
Altmetric has tracked 12,519,627 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,804 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 121,783 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.