Title |
Fluorescence resonance energy transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria
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Published in |
Biotechnology for Biofuels and Bioproducts, June 2008
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DOI | 10.1186/1754-6834-1-11 |
Pubmed ID | |
Authors |
Thijs Kaper, Ida Lager, Loren L Looger, Diane Chermak, Wolf B Frommer |
Abstract |
Engineering microorganisms to improve metabolite flux requires detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo. Fluorescence resonance energy transfer sensors represent a promising technology for measuring metabolite levels and corresponding rate changes in live cells. These sensors have been applied successfully in mammalian and plant cells but potentially could also be used to monitor steady-state levels of metabolites in microorganisms using fluorimetric assays. Sensors for hexose and pentose carbohydrates could help in the development of fermentative microorganisms, for example, for biofuels applications. Arabinose is one of the carbohydrates to be monitored during biofuels production from lignocellulose, while maltose is an important degradation product of starch that is relevant for starch-derived biofuels production. |
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United States | 1 | 25% |
Ireland | 1 | 25% |
Demographic breakdown
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Mendeley readers
Geographical breakdown
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United States | 2 | 2% |
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Brazil | 1 | <1% |
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Indonesia | 1 | <1% |
Singapore | 1 | <1% |
Other | 3 | 3% |
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Demographic breakdown
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Student > Ph. D. Student | 23 | 20% |
Student > Master | 19 | 17% |
Student > Bachelor | 9 | 8% |
Professor | 8 | 7% |
Other | 13 | 12% |
Unknown | 9 | 8% |
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