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Angiogenic factors are increased in circulating granulocytes and CD34+cells of myeloproliferative neoplasms

Overview of attention for article published in Molecular Carcinogenesis, July 2016
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Title
Angiogenic factors are increased in circulating granulocytes and CD34+cells of myeloproliferative neoplasms
Published in
Molecular Carcinogenesis, July 2016
DOI 10.1002/mc.22517
Pubmed ID
Authors

Tijana Subotički, Olivera Mitrović Ajtić, Bojana B. Beleslin-Čokić, Ronny Nienhold, Miloš Diklić, Dragoslava Djikić, Danijela Leković, Tanja Bulat, Dragana Marković, Mirjana Gotić, Constance T. Noguchi, Alan N. Schechter, Radek C. Skoda, Vladan P. Čokić

Abstract

It has been shown that angiogenesis and inflammation play an important role in development of most hematological malignancies including the myeloproliferative neoplasm (MPN). The aim of this study was to investigate and correlate the levels of key angiogenic molecules such as hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) in peripheral blood and bone marrow cells of MPN patients, along with JAK2V617F mutation allele burden and effects of therapy. HIF-1α and VEGF gene expression were decreased, while eNOS mRNA levels were increased in granulocytes of MPN patients. Furthermore, positively correlated and increased VEGF and eNOS protein levels were in negative correlation with HIF-1α levels in granulocytes of MPN patients. According to immunoblotting, the generally augmented angiogenic factors demonstrated JAK2V617F allele burden dependence only in granulocytes of PMF. The angiogenic factors were largely reduced after hydroxyurea therapy in granulocytes of MPN patients. Levels of eNOS protein expression were stimulated by Calreticulin mutations in granulocytes of essential thrombocythemia. Immunocytochemical analyses of CD34(+) cells showed a more pronounced enhancement of angiogenic factors than in granulocytes. Increased gene expression linked to the proinflammatory TGFβ and MAPK signaling pathways were detected in CD34(+) cells of MPN patients. In conclusion, the angiogenesis is increased in several cell types of MPN patients supported by the transcriptional activation of inflammation-related target genes, and is not limited to bone marrow stroma cells. It also appears that some of the benefit of hydroxyurea therapy of the MPN is mediated by effects on angiogenic factors. This article is protected by copyright. All rights reserved.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 23%
Student > Ph. D. Student 5 17%
Other 3 10%
Student > Doctoral Student 3 10%
Student > Master 2 7%
Other 5 17%
Unknown 5 17%
Readers by discipline Count As %
Medicine and Dentistry 10 33%
Biochemistry, Genetics and Molecular Biology 7 23%
Agricultural and Biological Sciences 3 10%
Immunology and Microbiology 2 7%
Unspecified 1 3%
Other 2 7%
Unknown 5 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 June 2016.
All research outputs
#9,738,146
of 12,181,787 outputs
Outputs from Molecular Carcinogenesis
#712
of 1,029 outputs
Outputs of similar age
#189,590
of 270,415 outputs
Outputs of similar age from Molecular Carcinogenesis
#20
of 32 outputs
Altmetric has tracked 12,181,787 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,029 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
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We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.