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Transcriptome analysis of primary podocytes reveals novel calcium regulated regulatory networks

Overview of attention for article published in FASEB Journal, September 2020
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Title
Transcriptome analysis of primary podocytes reveals novel calcium regulated regulatory networks
Published in
FASEB Journal, September 2020
DOI 10.1096/fj.201902493rr
Pubmed ID
Authors

Thaddäus Struk, Viji Nair, Felix Eichinger, Matthias Kretzler, Roland Wedlich‐Söldner, Samet Bayraktar, Hermann Pavenstädt

Abstract

Podocytes are pivotal in establishing the selective permeability of the glomerular filtration barrier. Recently, we showed that an increase of the intracellular calcium ion concentration [Ca2+ ] causes a rapid and transient actin reset (CaAR) measurable through live imaging microscopy using lifeact-mCherry as an actin dye in different cell types including the podocyte. This and other studies show the critical role [Ca2+ ] and the actin cytoskeleton play in podocyte homeostasis. To further investigate the role of [Ca2+ ] and the actin cytoskeleton in podocytes, we used a double fluorescent reporter mouse model to establish a primary podocyte culture system. We treated these podocytes temporarily with a Calcium Ionophore and facultatively with Latrunculin A, an inhibitor of actin polymerization. Unbiased genome wide transcriptional analysis identified a transcriptional response in podocytes to elevated [Ca2+ ] levels, affecting mRNA levels of PDGF-BB, RICTOR, and MIR17HG as mediators of Ca2+ -signaling. Comparison of the ex vivo transcriptional response from the primary podocyte culture with glomerular transcripts across a wide spectrum of CKD disease confirmed co-regulation of transcript sets, establishing the disease relevance of the model system. Our findings demonstrate novel [Ca2+ ] regulated gene networks in podocytes deepening our understanding of podocyte biology and disease.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 63%
Researcher 1 13%
Student > Doctoral Student 1 13%
Student > Master 1 13%
Readers by discipline Count As %
Medicine and Dentistry 3 38%
Agricultural and Biological Sciences 2 25%
Biochemistry, Genetics and Molecular Biology 1 13%
Physics and Astronomy 1 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 13%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2020.
All research outputs
#16,719,819
of 18,905,383 outputs
Outputs from FASEB Journal
#7,547
of 8,382 outputs
Outputs of similar age
#269,214
of 319,129 outputs
Outputs of similar age from FASEB Journal
#156
of 187 outputs
Altmetric has tracked 18,905,383 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,382 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 187 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.