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Silver nanoparticles for the detection of cell surface antigens in mass cytometry

Overview of attention for article published in Cytometry Part A, June 2016
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  • Above-average Attention Score compared to outputs of the same age (58th percentile)
  • Average Attention Score compared to outputs of the same age and source

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4 tweeters

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10 Mendeley
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Title
Silver nanoparticles for the detection of cell surface antigens in mass cytometry
Published in
Cytometry Part A, June 2016
DOI 10.1002/cyto.a.22904
Pubmed ID
Authors

Schulz, Axel Ronald, Stanislawiak, Silke, Baumgart, Sabine, Grützkau, Andreas, Mei, Henrik E, Mei, Henrik E.

Abstract

Mass cytometry has pioneered >40-parameter single-cell analyses that allow for the characterization of complex cellular networks at unprecedented depth. Up to 135 parameters can be simultaneously detected, but limited availability of metal tags suitable for labeling of specific probes prevents optimal exploitation of the analytical capacity of mass cytometers. To this end, we here establish the application of elemental silver nanoparticles (AgNP) of different size for reporting cell surface antigens on human leukocytes in mass cytometry assays. The mass channels at 107 Da and 109 Da are uniquely occupied by silver isotopes and do not interfere with other mass cytometry reagents. Streptavidin-coated AgNP (SA-AgNP) facilitated distinct and specific detection of various antigens, such as CD8, CD244 and CD294 on peripheral blood leukocytes pre-incubated with respective biotinylated primary antibodies. Signal intensities elicited by 40 nm-sized AgNP allowed specific detection of the low abundance antigen CD25 on both, peripheral blood regulatory T cells and CD25(lo) CD127(+) CD4(+) T cells, enabling their distinct clustering in viSNE plots. SA-AgNP were of high elemental purity, showed minor background binding to cells in immunoassays, and were compatible with previously established staining protocols for PBMC and leukocytes, facilitating their use in complex mass cytometry panels. Considering the synthesis of AgNP from isotopically purified silver, the usage of AgNP extends the analytical capacity of mass cytometry panels by one, prospectively two, additional parameters, suitable for the detection of cellular targets of low abundance. © 2016 International Society for Advancement of Cytometry.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Student > Master 2 20%
Student > Ph. D. Student 2 20%
Professor > Associate Professor 2 20%
Student > Postgraduate 1 10%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 50%
Unspecified 1 10%
Biochemistry, Genetics and Molecular Biology 1 10%
Nursing and Health Professions 1 10%
Immunology and Microbiology 1 10%
Other 1 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 November 2016.
All research outputs
#3,814,337
of 8,683,790 outputs
Outputs from Cytometry Part A
#197
of 470 outputs
Outputs of similar age
#106,517
of 265,766 outputs
Outputs of similar age from Cytometry Part A
#10
of 15 outputs
Altmetric has tracked 8,683,790 research outputs across all sources so far. This one has received more attention than most of these and is in the 54th percentile.
So far Altmetric has tracked 470 research outputs from this source. They receive a mean Attention Score of 3.0. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,766 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.