Title |
PhyloToAST: Bioinformatics tools for species-level analysis and visualization of complex microbial datasets
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Published in |
Scientific Reports, June 2016
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DOI | 10.1038/srep29123 |
Pubmed ID | |
Authors |
Shareef M. Dabdoub, Megan L. Fellows, Akshay D. Paropkari, Matthew R. Mason, Sarandeep S. Huja, Alexandra A. Tsigarida, Purnima S. Kumar |
Abstract |
The 16S rRNA gene is widely used for taxonomic profiling of microbial ecosystems; and recent advances in sequencing chemistry have allowed extremely large numbers of sequences to be generated from minimal amounts of biological samples. Analysis speed and resolution of data to species-level taxa are two important factors in large-scale explorations of complex microbiomes using 16S sequencing. We present here new software, Phylogenetic Tools for Analysis of Species-level Taxa (PhyloToAST), that completely integrates with the QIIME pipeline to improve analysis speed, reduce primer bias (requiring two sequencing primers), enhance species-level analysis, and add new visualization tools. The code is free and open source, and can be accessed at http://phylotoast.org. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 10 | 37% |
France | 2 | 7% |
Canada | 2 | 7% |
Australia | 1 | 4% |
Ireland | 1 | 4% |
Finland | 1 | 4% |
Chile | 1 | 4% |
Spain | 1 | 4% |
Mexico | 1 | 4% |
Other | 0 | 0% |
Unknown | 7 | 26% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 15 | 56% |
Scientists | 12 | 44% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
Switzerland | 1 | <1% |
Netherlands | 1 | <1% |
Ireland | 1 | <1% |
Canada | 1 | <1% |
Mexico | 1 | <1% |
United States | 1 | <1% |
Unknown | 102 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 23 | 21% |
Researcher | 23 | 21% |
Student > Master | 15 | 14% |
Student > Bachelor | 12 | 11% |
Professor | 4 | 4% |
Other | 12 | 11% |
Unknown | 20 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 37 | 34% |
Biochemistry, Genetics and Molecular Biology | 18 | 17% |
Environmental Science | 6 | 6% |
Computer Science | 5 | 5% |
Medicine and Dentistry | 4 | 4% |
Other | 14 | 13% |
Unknown | 25 | 23% |