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Molecular detection of airborne Coccidioides in Tucson, Arizona

Overview of attention for article published in Medical Mycology, May 2016
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  • Good Attention Score compared to outputs of the same age (69th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

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Citations

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39 Mendeley
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Title
Molecular detection of airborne Coccidioides in Tucson, Arizona
Published in
Medical Mycology, May 2016
DOI 10.1093/mmy/myw022
Pubmed ID
Authors

Nancy A Chow, Dale W Griffin, Bridget M Barker, Vladimir N Loparev, Anastasia P Litvintseva

Abstract

Environmental surveillance of the soil-dwelling fungus Coccidioides is essential for the prevention of Valley fever, a disease primarily caused by inhalation of the arthroconidia. Methods for collecting and detecting Coccidioides in soil samples are currently in use by several laboratories; however, a method utilizing current air sampling technologies has not been formally demonstrated for the capture of airborne arthroconidia. In this study, we collected air/dust samples at two sites (Site A and Site B) in the endemic region of Tucson, Arizona, and tested a variety of air samplers and membrane matrices. We then employed a single-tube nested qPCR assay for molecular detection. At both sites, numerous soil samples (n = 10 at Site A and n = 24 at Site B) were collected and Coccidioides was detected in two samples (20%) at Site A and in eight samples (33%) at Site B. Of the 25 air/dust samples collected at both sites using five different air sampling methods, we detected Coccidioides in three samples from site B. All three samples were collected using a high-volume sampler with glass-fiber filters. In this report, we describe these methods and propose the use of these air sampling and molecular detection strategies for environmental surveillance of Coccidioides.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 38 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 15%
Student > Master 5 13%
Student > Doctoral Student 4 10%
Student > Bachelor 4 10%
Other 3 8%
Other 7 18%
Unknown 10 26%
Readers by discipline Count As %
Immunology and Microbiology 6 15%
Agricultural and Biological Sciences 5 13%
Biochemistry, Genetics and Molecular Biology 5 13%
Medicine and Dentistry 3 8%
Environmental Science 2 5%
Other 6 15%
Unknown 12 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2017.
All research outputs
#6,623,520
of 23,577,654 outputs
Outputs from Medical Mycology
#254
of 1,367 outputs
Outputs of similar age
#92,173
of 300,417 outputs
Outputs of similar age from Medical Mycology
#1
of 19 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,367 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,417 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.