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Mesenchymal Stem Cells

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Cover of 'Mesenchymal Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Mesenchymal Stem Cells
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    Chapter 2 Bone Tissue Engineering: Past-Present-Future.
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    Chapter 3 Mesenchymal Stem Cells for Osteochondral Tissue Engineering.
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    Chapter 4 Mesenchymal Stem Cells
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    Chapter 5 Mesenchymal Stem Cells
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    Chapter 6 Mesenchymal Stem Cells in Lipogems, a Reverse Story: from Clinical Practice to Basic Science.
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    Chapter 7 Paracrine Mechanisms of Mesenchymal Stem Cells in Tissue Repair.
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    Chapter 8 Mesenchymal Stem Cells
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    Chapter 9 Colony Forming Unit Assays.
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    Chapter 10 Methods and Strategies for Lineage Tracing of Mesenchymal Progenitor Cells.
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    Chapter 11 Mesenchymal Stem Cells
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    Chapter 12 Mesenchymal Stem Cells
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    Chapter 13 Mesenchymal Stem Cells
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    Chapter 14 Mesenchymal Stem Cells
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    Chapter 15 Isolation, Expansion, and Immortalization of Human Adipose-Derived Mesenchymal Stromal Cells from Biopsies and Liposuction Specimens.
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    Chapter 16 Optimization of Mesenchymal Stem Cells to Increase Their Therapeutic Potential.
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    Chapter 17 Mesenchymal Stem Cells
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    Chapter 18 Isolation and Manufacture of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells.
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    Chapter 19 Quality Control Assays for Clinical-Grade Human Mesenchymal Stromal Cells: Methods for ATMP Release.
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    Chapter 20 Quality Control Assays for Clinical-Grade Human Mesenchymal Stromal Cells: Validation Strategy.
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    Chapter 21 Mesenchymal Stem Cells
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    Chapter 22 Clinical-Grade Manufacturing of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems.
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    Chapter 23 GMP-Compliant Expansion of Clinical-Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow Fiber Bioreactor.
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    Chapter 24 Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells.
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    Chapter 25 Fabrication of Elasticity-Tunable Gelatinous Gel for Mesenchymal Stem Cell Culture.
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    Chapter 26 Mesenchymal Stem Cells
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    Chapter 27 Tips on How to Collect and Administer the Mesenchymal Stem Cell Secretome for Central Nervous System Applications.
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    Chapter 28 Soluble Factors from Human Fetal Bone Marrow-Derived Mesenchymal Stem Cells: Preparation of Conditioned Medium and Its Effect on Tumor Cells.
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    Chapter 29 Mesenchymal Stem Cells
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    Chapter 30 Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells.
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    Chapter 31 Proteomic Analysis of Mesenchymal Stem Cells.
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    Chapter 32 Unraveling Mesenchymal Stem Cells' Dynamic Secretome Through Nontargeted Proteomics Profiling.
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    Chapter 33 Identification of Factors Produced and Secreted by Mesenchymal Stromal Cells with the SILAC Method.
Attention for Chapter 20: Quality Control Assays for Clinical-Grade Human Mesenchymal Stromal Cells: Validation Strategy.
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Chapter title
Quality Control Assays for Clinical-Grade Human Mesenchymal Stromal Cells: Validation Strategy.
Chapter number 20
Book title
Mesenchymal Stem Cells
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3584-0_20
Pubmed ID
27236682
Book ISBNs
978-1-4939-3582-6, 978-1-4939-3584-0
Authors

Marina Radrizzani, Sabrina Soncin, Sara Bolis, Viviana Lo Cicero, Gabriella Andriolo, Lucia Turchetto

Editors

Massimiliano Gnecchi

Abstract

The present chapter focuses on the validation of the following analytical methods for the control of mesenchymal stromal cells (MSC) for cell therapy clinical trials: Microbiological control for cellular product Endotoxin assay Mycoplasma assay Cell count and viability Immunophenotype Clonogenic potential (CFU-F assay) In our lab, these methods are in use for product release, process control or control of the biological starting materials. They are described in detail in the accompanying Chapter 19.For each method, validation goals and strategy are presented, and a detailed experimental scheme is proposed.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 21%
Student > Ph. D. Student 5 18%
Student > Master 4 14%
Student > Bachelor 2 7%
Other 2 7%
Other 2 7%
Unknown 7 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 18%
Biochemistry, Genetics and Molecular Biology 4 14%
Engineering 4 14%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Medicine and Dentistry 2 7%
Other 3 11%
Unknown 8 29%

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