| Chapter title |
Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells.
|
|---|---|
| Chapter number | 24 |
| Book title |
Mesenchymal Stem Cells
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3584-0_24 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3582-6, 978-1-4939-3584-0
|
| Authors |
Celeste Scotti, Beatrice Tonnarelli, Adam Papadimitropoulos, Elia Piccinini, Atanas Todorov, Matteo Centola, Andrea Barbero, Ivan Martin |
| Editors |
Massimiliano Gnecchi |
| Abstract |
Bone development, growth, and repair predominantly occur through the process of endochondral ossification, characterized by remodelling of cartilaginous templates. The same route efficiently supports engineering of bone marrow as a niche for hematopoietic stem cells (HSC). Here we describe a combined in vitro/in vivo system based on bone marrow-derived Mesenchymal Stem/Stromal Cells (MSC) that duplicates the hallmark cellular and molecular events of endochondral ossification during development. The model requires MSC culture with instructive molecules to generate hypertrophic cartilage tissues. The resulting constructs complete the endochondral route upon in vivo implantation, in the timeframe of up to 12 weeks. The described protocol is clearly distinct from the direct ossification approach typically used to drive MSC towards osteogenesis. Recapitulation of endochondral ossification allows modelling of stromal-HSC interactions in physiology and pathology and allows engineering processes underlying bone regeneration. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 53 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 26% |
| Student > Ph. D. Student | 10 | 19% |
| Student > Master | 8 | 15% |
| Student > Bachelor | 6 | 11% |
| Professor | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 9 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 11 | 21% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Engineering | 5 | 9% |
| Agricultural and Biological Sciences | 5 | 9% |
| Immunology and Microbiology | 3 | 6% |
| Other | 10 | 19% |
| Unknown | 11 | 21% |