Chapter title |
Fabrication of Elasticity-Tunable Gelatinous Gel for Mesenchymal Stem Cell Culture.
|
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Chapter number | 25 |
Book title |
Mesenchymal Stem Cells
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3584-0_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3582-6, 978-1-4939-3584-0
|
Authors |
Thasaneeya Kuboki, Satoru Kidoaki |
Editors |
Massimiliano Gnecchi |
Abstract |
Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 49 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 2% |
Student > Doctoral Student | 1 | 2% |
Unknown | 47 | 96% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 1 | 2% |
Unknown | 48 | 98% |