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Mesenchymal Stem Cells

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Cover of 'Mesenchymal Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Mesenchymal Stem Cells
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    Chapter 2 Bone Tissue Engineering: Past-Present-Future.
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    Chapter 3 Mesenchymal Stem Cells for Osteochondral Tissue Engineering.
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    Chapter 4 Mesenchymal Stem Cells
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    Chapter 5 Mesenchymal Stem Cells
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    Chapter 6 Mesenchymal Stem Cells in Lipogems, a Reverse Story: from Clinical Practice to Basic Science.
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    Chapter 7 Paracrine Mechanisms of Mesenchymal Stem Cells in Tissue Repair.
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    Chapter 8 Mesenchymal Stem Cells
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    Chapter 9 Colony Forming Unit Assays.
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    Chapter 10 Methods and Strategies for Lineage Tracing of Mesenchymal Progenitor Cells.
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    Chapter 11 Mesenchymal Stem Cells
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    Chapter 12 Mesenchymal Stem Cells
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    Chapter 13 Mesenchymal Stem Cells
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    Chapter 14 Mesenchymal Stem Cells
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    Chapter 15 Isolation, Expansion, and Immortalization of Human Adipose-Derived Mesenchymal Stromal Cells from Biopsies and Liposuction Specimens.
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    Chapter 16 Optimization of Mesenchymal Stem Cells to Increase Their Therapeutic Potential.
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    Chapter 17 Mesenchymal Stem Cells
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    Chapter 18 Isolation and Manufacture of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells.
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    Chapter 19 Quality Control Assays for Clinical-Grade Human Mesenchymal Stromal Cells: Methods for ATMP Release.
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    Chapter 20 Quality Control Assays for Clinical-Grade Human Mesenchymal Stromal Cells: Validation Strategy.
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    Chapter 21 Mesenchymal Stem Cells
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    Chapter 22 Clinical-Grade Manufacturing of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems.
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    Chapter 23 GMP-Compliant Expansion of Clinical-Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow Fiber Bioreactor.
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    Chapter 24 Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells.
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    Chapter 25 Fabrication of Elasticity-Tunable Gelatinous Gel for Mesenchymal Stem Cell Culture.
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    Chapter 26 Mesenchymal Stem Cells
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    Chapter 27 Tips on How to Collect and Administer the Mesenchymal Stem Cell Secretome for Central Nervous System Applications.
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    Chapter 28 Soluble Factors from Human Fetal Bone Marrow-Derived Mesenchymal Stem Cells: Preparation of Conditioned Medium and Its Effect on Tumor Cells.
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    Chapter 29 Mesenchymal Stem Cells
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    Chapter 30 Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells.
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    Chapter 31 Proteomic Analysis of Mesenchymal Stem Cells.
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    Chapter 32 Unraveling Mesenchymal Stem Cells' Dynamic Secretome Through Nontargeted Proteomics Profiling.
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    Chapter 33 Identification of Factors Produced and Secreted by Mesenchymal Stromal Cells with the SILAC Method.
Attention for Chapter 30: Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells.
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Chapter title
Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells.
Chapter number 30
Book title
Mesenchymal Stem Cells
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3584-0_30
Pubmed ID
Book ISBNs
978-1-4939-3582-6, 978-1-4939-3584-0
Authors

Jose Gomez, Jeffrey Schmeckpeper, Maria Mirotsou

Editors

Massimiliano Gnecchi

Abstract

Mesenchymal stem cells (MSC) from bone marrow or adult tissues are widely studied to evaluate their potential for tissue repair. Differences in tissue of origin, donor variation, or in vitro handling exist and it is still unclear how they affect cell function and regenerative potential. Large-scale gene expression analysis of these cells not only allows researchers to compare and contrast the differences between each MSC subset but also allows for the development of better analytical tools for their characterization and utilization. Here, we describe a protocol for transcriptomics analysis of MSC-like cells derived from adult kidneys.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 33%
Unknown 2 67%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Unknown 2 67%