Title |
Genome wide identification of chilling responsive microRNAs in Prunus persica
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Published in |
BMC Genomics, September 2012
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DOI | 10.1186/1471-2164-13-481 |
Pubmed ID | |
Authors |
Abdelali Barakat, Aditya Sriram, Joseph Park, Tetyana Zhebentyayeva, Dorrie Main, Albert Abbott |
Abstract |
MicroRNAs (miRNAs) are small RNAs (sRNAs) approximately 21 nucleotides in length that negatively control gene expression by cleaving or inhibiting the translation of target gene transcripts. Within this context, miRNAs and siRNAs are coming to the forefront as molecular mediators of gene regulation in plant responses to annual temperature cycling and cold stress. For this reason, we chose to identify and characterize the conserved and non-conserved miRNA component of peach (Prunus persica (L.) Batsch) focusing our efforts on both the recently released whole genome sequence of peach and sRNA transcriptome sequences from two tissues representing non-dormant leaves and dormant leaf buds. Conserved and non-conserved miRNAs, and their targets were identified. These sRNA resources were used to identify cold-responsive miRNAs whose gene targets co-localize with previously described QTLs for chilling requirement (CR). |
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