Title |
Illegitimate recombination: An efficient method for random mutagenesis in Mycobacterium avium subsp. hominissuis
|
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Published in |
BMC Microbiology, September 2012
|
DOI | 10.1186/1471-2180-12-204 |
Pubmed ID | |
Authors |
Faisal Asghar Khattak, Ashutosh Kumar, Elisabeth Kamal, Ralph Kunisch, Astrid Lewin |
Abstract |
The genus Mycobacterium (M.) comprises highly pathogenic bacteria such as M. tuberculosis as well as environmental opportunistic bacteria called non-tuberculous mycobacteria (NTM). While the incidence of tuberculosis is declining in the developed world, infection rates by NTM are increasing. NTM are ubiquitous and have been isolated from soil, natural water sources, tap water, biofilms, aerosols, dust and sawdust. Lung infections as well as lymphadenitis are most often caused by M. avium subsp. hominissuis (MAH), which is considered to be among the clinically most important NTM. Only few virulence genes from M. avium have been defined among other things due to difficulties in generating M. avium mutants. More efforts in developing new methods for mutagenesis of M. avium and identification of virulence-associated genes are therefore needed. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 2% |
Ghana | 1 | 2% |
Unknown | 45 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 23% |
Student > Master | 9 | 19% |
Student > Ph. D. Student | 8 | 17% |
Other | 2 | 4% |
Student > Doctoral Student | 1 | 2% |
Other | 4 | 9% |
Unknown | 12 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 23% |
Immunology and Microbiology | 9 | 19% |
Biochemistry, Genetics and Molecular Biology | 5 | 11% |
Medicine and Dentistry | 5 | 11% |
Veterinary Science and Veterinary Medicine | 2 | 4% |
Other | 0 | 0% |
Unknown | 15 | 32% |