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Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers

Overview of attention for article published in Brazilian Journal of Infectious Diseases, May 2016
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1 tweeter

Citations

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Readers on

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15 Mendeley
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Title
Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers
Published in
Brazilian Journal of Infectious Diseases, May 2016
DOI 10.1016/j.bjid.2016.01.003
Pubmed ID
Authors

Luiz Henrique Soares Tibo, Jéssica Wildgrube Bertol, Robert Alvin Bernedo-Navarro, Tomomasa Yano

Abstract

Culture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup O153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of ∼150kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40μg/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's permeabilizing factor), may be an important SEPEC virulence factor that facilitates bacterial access to the bloodstream.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 20%
Student > Postgraduate 2 13%
Student > Bachelor 2 13%
Student > Ph. D. Student 2 13%
Student > Master 1 7%
Other 2 13%
Unknown 3 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 27%
Agricultural and Biological Sciences 3 20%
Medicine and Dentistry 2 13%
Nursing and Health Professions 1 7%
Immunology and Microbiology 1 7%
Other 1 7%
Unknown 3 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 July 2016.
All research outputs
#12,603,341
of 15,869,780 outputs
Outputs from Brazilian Journal of Infectious Diseases
#285
of 481 outputs
Outputs of similar age
#184,885
of 263,425 outputs
Outputs of similar age from Brazilian Journal of Infectious Diseases
#5
of 7 outputs
Altmetric has tracked 15,869,780 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 481 research outputs from this source. They receive a mean Attention Score of 2.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,425 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.