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Platelet-derived extracellular vesicles promote osteoinduction of mesenchymal stromal cells

Overview of attention for article published in Bone and Joint Research, October 2020
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (70th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (53rd percentile)

Mentioned by

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9 tweeters
facebook
1 Facebook page

Citations

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7 Dimensions

Readers on

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28 Mendeley
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Title
Platelet-derived extracellular vesicles promote osteoinduction of mesenchymal stromal cells
Published in
Bone and Joint Research, October 2020
DOI 10.1302/2046-3758.910.bjr-2020-0111.r2
Pubmed ID
Authors

Miquel Antich-Rosselló, Maria Antònia Forteza-Genestra, Javier Calvo, Antoni Gayà, Marta Monjo, Joana M. Ramis

Abstract

Platelet concentrates, like platelet-rich plasma (PRP) and platelet lysate (PL), are widely used in regenerative medicine, especially in bone regeneration. However, the lack of standard procedures and controls leads to high variability in the obtained results, limiting their regular clinical use. Here, we propose the use of platelet-derived extracellular vesicles (EVs) as an off-the-shelf alternative for PRP and PL for bone regeneration. In this article, we evaluate the effect of PL-derived EVs on the biocompatibility and differentiation of mesenchymal stromal cells (MSCs). EVs were obtained first by ultracentrifugation (UC) and then by size exclusion chromatography (SEC) from non-activated PL. EVs were characterized by transmission electron microscopy, nanoparticle tracking analysis, and the expression of CD9 and CD63 markers by western blot. The effect of the obtained EVs on osteoinduction was evaluated in vitro on human umbilical cord MSCs by messenger RNA (mRNA) expression analysis of bone markers, alkaline phosphatase activity (ALP), and calcium (Ca2+) content. Osteogenic differentiation of MSCs was confirmed when treated with UC-isolated EVs. In order to disprove that the effect was due to co-isolated proteins, EVs were isolated by SEC. Purer EVs were obtained and proved to maintain the differentiation effect on MSCs and showed a dose-dependent response. PL-derived EVs present an osteogenic capability comparable to PL treatments, emerging as an alternative able to overcome PL and PRP limitations.Cite this article: Bone Joint Res 2020;9(10):667-674.

Twitter Demographics

The data shown below were collected from the profiles of 9 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 32%
Researcher 4 14%
Student > Bachelor 2 7%
Student > Master 2 7%
Student > Doctoral Student 1 4%
Other 3 11%
Unknown 7 25%
Readers by discipline Count As %
Medicine and Dentistry 9 32%
Biochemistry, Genetics and Molecular Biology 6 21%
Engineering 3 11%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Materials Science 1 4%
Other 0 0%
Unknown 7 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 September 2021.
All research outputs
#5,014,238
of 20,423,840 outputs
Outputs from Bone and Joint Research
#311
of 683 outputs
Outputs of similar age
#142,435
of 483,893 outputs
Outputs of similar age from Bone and Joint Research
#12
of 26 outputs
Altmetric has tracked 20,423,840 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 683 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.8. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 483,893 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.