Title |
Increased knowledge of Francisellagenus diversity highlights the benefits of optimised DNA-based assays
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Published in |
BMC Microbiology, September 2012
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DOI | 10.1186/1471-2180-12-220 |
Pubmed ID | |
Authors |
Jon Ahlinder, Caroline Öhrman, Kerstin Svensson, Petter Lindgren, Anders Johansson, Mats Forsman, Pär Larsson, Andreas Sjödin |
Abstract |
Recent advances in sequencing technologies offer promising tools for generating large numbers of genomes, larger typing databases and improved mapping of environmental bacterial diversity. However, DNA-based methods for the detection of Francisella were developed with limited knowledge about genetic diversity. This, together with the high sequence identity between several Francisella species, means there is a high risk of false identification and detection of the highly virulent pathogen Francisella tularensis. Moreover, phylogenetic reconstructions using single or limited numbers of marker sequences often result in incorrect tree topologies and inferred evolutionary distances. The recent growth in publicly accessible whole-genome sequences now allows evaluation of published genetic markers to determine optimal combinations of markers that minimise both time and laboratory costs. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Sweden | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Sweden | 2 | 4% |
Colombia | 1 | 2% |
Unknown | 42 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 15 | 33% |
Student > Ph. D. Student | 9 | 20% |
Student > Master | 5 | 11% |
Professor > Associate Professor | 4 | 9% |
Student > Doctoral Student | 3 | 7% |
Other | 2 | 4% |
Unknown | 7 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 20 | 44% |
Immunology and Microbiology | 5 | 11% |
Biochemistry, Genetics and Molecular Biology | 4 | 9% |
Environmental Science | 2 | 4% |
Engineering | 2 | 4% |
Other | 3 | 7% |
Unknown | 9 | 20% |