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Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood

Overview of attention for article published in BMC Veterinary Research, September 2012
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  • Above-average Attention Score compared to outputs of the same age and source (61st percentile)

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Title
Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood
Published in
BMC Veterinary Research, September 2012
DOI 10.1186/1746-6148-8-169
Pubmed ID
Authors

Jaber Lyahyai, Diego R Mediano, Beatriz Ranera, Arianne Sanz, Ana Rosa Remacha, Rosa Bolea, Pilar Zaragoza, Clementina Rodellar, Inmaculada Martín-Burriel

Abstract

Mesenchymal stem cells (MSCs) are multipotent stem cells with capacity to differentiate into several mesenchymal lineages. This quality makes MSCs good candidates for use in cell therapy. MSCs can be isolated from a variety of tissues including bone marrow and adipose tissue, which are the most common sources of these cells. However, MSCs can also be isolated from peripheral blood. Sheep has been proposed as an ideal model for biomedical studies including those of orthopaedics and transmissible spongiform encephalopathies (TSEs). The aim of this work was to advance these studies by investigating the possibility of MSC isolation from ovine peripheral blood (oPB-MSCs) and by subsequently characterizing there in vitro properties. Plastic-adherent fibroblast-like cells were obtained from the mononuclear fraction of blood samples. These cells were analysed for their proliferative and differentiation potential into adipocytes, osteoblasts and chondrocytes, as well as for the gene expression of cell surface markers. The isolated cells expressed transcripts for markers CD29, CD73 and CD90, but failed to express the haematopoietic marker CD45 and expressed only low levels of CD105. The expression of CD34 was variable. The differentiation potential of this cell population was evaluated using specific differentiation media. Although the ability of the cultures derived from different animals to differentiate into adipocytes, osteoblasts and chondrocytes was heterogeneous, we confirmed this feature using specific staining and analysing the gene expression of differentiation markers. Finally, we tested the ability of oPB-MSCs to transdifferentiate into neuronal-like cells. Morphological changes were observed after 24-hour culture in neurogenic media, and the transcript levels of the neurogenic markers increased during the prolonged induction period. Moreover, oPB-MSCs expressed the cellular prion protein gene (PRNP), which was up-regulated during neurogenesis. This study describes for the first time the isolation and characterization of oPB-MSCs. Albeit some variability was observed between animals, these cells retained their capacity to differentiate into mesenchymal lineages and to transdifferentiate into neuron-like cells in vitro. Therefore, oPB-MSCs could serve as a valuable tool for biomedical research in fields including orthopaedics or prion diseases.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 1 1%
Unknown 88 99%

Demographic breakdown

Readers by professional status Count As %
Student > Master 17 19%
Student > Ph. D. Student 14 16%
Researcher 13 15%
Student > Bachelor 12 13%
Professor > Associate Professor 4 4%
Other 13 15%
Unknown 16 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 19 21%
Medicine and Dentistry 17 19%
Agricultural and Biological Sciences 15 17%
Veterinary Science and Veterinary Medicine 4 4%
Engineering 3 3%
Other 9 10%
Unknown 22 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2013.
All research outputs
#14,089,487
of 22,679,690 outputs
Outputs from BMC Veterinary Research
#1,080
of 3,033 outputs
Outputs of similar age
#97,973
of 170,921 outputs
Outputs of similar age from BMC Veterinary Research
#14
of 36 outputs
Altmetric has tracked 22,679,690 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,033 research outputs from this source. They receive a mean Attention Score of 3.8. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 170,921 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.