Title |
Amyloid-β peptide absence in short term effects on kinase activity of energy metabolism in mice hippocampus and cerebral cortex
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Published in |
Anais da Academia Brasileira de Ciências, July 2016
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DOI | 10.1590/0001-3765201620150776 |
Pubmed ID | |
Authors |
Francine R Ianiski, Virginia C Rech, Vivian S K Nishihira, Catiane B Alves, Matheus D Baldissera, Ethel A Wilhelm, Cristiane Luchese |
Abstract |
Considering that Alzheimer's disease is a prevalent neurodegenerative disease worldwide, we investigated the activities of three key kinases: creatine kinase, pyruvate kinase and adenylate kinase in the hippocampus and cerebral cortex in Alzheimer's disease model. Male adult Swiss mice received amyloid-β or saline. One day after, mice were treated with blank nanocapsules (17 ml/kg) or meloxicam-loaded nanocapsules (5 mg/kg) or free meloxicam (5 mg/kg). Treatments were performed on alternating days, until the end of the experimental protocol. In the fourteenth day, kinases activities were performed. Amyloid-β did not change the kinases activity in the hippocampus and cerebral cortex of mice. However, free meloxicam decrease the creatine kinase activity in mitochondrial-rich fraction in the group induced by amyloid-β, but for the cytosolic fraction, it has raised in the activity of pyruvate kinase activity in cerebral cortex. Further, meloxicam-loaded nanocapsules administration reduced adenylate kinase activity in the hippocampus of mice injected by amyloid-β. In conclusion we observed absence in short-term effects in kinases activities of energy metabolism in mice hippocampus and cerebral cortex using amyloid-β peptide model. These findings established the foundation to further study the kinases in phosphoryltransfer network changes observed in the brains of patients post-mortem with Alzheimer's disease. |
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Unknown | 21 | 100% |
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Readers by professional status | Count | As % |
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Other | 2 | 10% |
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Researcher | 2 | 10% |
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Computer Science | 1 | 5% |
Other | 3 | 14% |
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