| Chapter title |
Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ.
|
|---|---|
| Chapter number | 9 |
| Book title |
Multipotent Stem Cells of the Hair Follicle
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3786-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3784-4, 978-1-4939-3786-8
|
| Authors |
Katsunari Tezuka, Koh-ei Toyoshima, Takashi Tsuji |
| Editors |
Robert M Hoffman |
| Abstract |
Hair follicle morphogenesis is first induced by epithelial-mesenchymal interactions in the developing embryo. In the hair follicle, various stem-cell populations are maintained in specialized niches to promote repetitive hair follicle-morphogenesis, which is observed in the variable lower region of the hair follicle as a postnatal hair cycle. In contrast, the genesis of most organs is induced only once during embryogenesis. We developed a novel bioengineering technique, the Organ Germ Method, that employs three-dimensional stem cell culture for regenerating various organs and reproducing embryonic organogenesis. In this chapter, we describe a protocol for hair follicle germ reconstitution using adult follicle-derived epithelial stem cells and dermal papilla cells with intracutaneous transplantation of the bioengineered hair-follicle organ germ. This protocol can be useful not only for the clinical study of hair regeneration but also for studies of stem cell biology and organogenesis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Student > Bachelor | 1 | 14% |
| Student > Master | 1 | 14% |
| Researcher | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 3 | 43% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Unknown | 1 | 14% |