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Multipotent Stem Cells of the Hair Follicle

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Cover of 'Multipotent Stem Cells of the Hair Follicle'

Table of Contents

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    Book Overview
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    Chapter 1 Multipotent Stem Cells of the Hair Follicle
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    Chapter 2 Nestin-Based Reporter Transgenic Mouse Lines.
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    Chapter 3 Discovery of HAP Stem Cells.
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    Chapter 4 Multipotent Stem Cells of the Hair Follicle
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    Chapter 5 Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells.
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    Chapter 6 Nestin-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam® Histoculture.
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    Chapter 7 Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles.
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    Chapter 8 Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay.
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    Chapter 9 Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ.
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    Chapter 10 Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation.
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    Chapter 11 Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.
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    Chapter 12 Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination.
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    Chapter 13 Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase.
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    Chapter 14 Protocols for Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice.
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    Chapter 15 Protocols for Gelfoam(®) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods.
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    Chapter 16 Multipotent Stem Cells of the Hair Follicle
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    Chapter 17 Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium.
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    Chapter 18 Multipotent Stem Cells of the Hair Follicle
Attention for Chapter 5: Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells.
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Chapter title
Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells.
Chapter number 5
Book title
Multipotent Stem Cells of the Hair Follicle
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3786-8_5
Pubmed ID
Book ISBNs
978-1-4939-3784-4, 978-1-4939-3786-8
Authors

Fang Liu, Haiyan Lin, Chuansen Zhang

Editors

Robert M Hoffman

Abstract

Tissue-engineered nerve conduits are widely used for the study of peripheral nerve injury repair. With regard to repairing long nerve defects, stem-cell-derived neurons are recommended as seed cells. As hair-follicle neural crest stem cells (hfNCSCs) are easily to be harvested from patients and have the potential to differentiate into neuronal cells, hfNCSCs-derived neurons are an ideal candidate choice. Acellular nerve grafts, a type of biological material scaffold, with intact collagen structure, with biocompatibility and less toxicity are obtained through removing live cells with 1 % lysolecithin, are also an ideal choice. In the present report, we describe a tissue-engineered nerve conduit seeded with rat hfNCSCs-derived neurons into the beagle acellular sciatic nerve scaffold. Our goal is to provide a novel engineered therapeutic for repairing peripheral nerve injury with long distance defects.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 40%
Researcher 2 40%
Student > Ph. D. Student 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 20%
Neuroscience 1 20%
Chemistry 1 20%
Unknown 2 40%