Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.
Multipotent Stem Cells of the Hair Follicle
Methods in molecular biology, January 2016
Eduardo Martínez-Martínez, Eileen Uribe-Querol, Claudio I. Galván-Hernández, Gabriel Gutiérrez-Ospina
Robert M Hoffman
We describe a stereology method to obtain reliable estimates of the total number of proliferative and migratory epithelial cells after wounding. Using pulse and chase experiments with halogenated thymidine analogs such as iododeoxyuridine (IdU) and chlorodeoxyuridine (CldU), it is possible to track epithelial populations with heterogeneous proliferative characteristics through skin compartments. The stereological and tissue processing methods described here apply widely to address important questions of skin stem-cell biology.
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