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Multipotent Stem Cells of the Hair Follicle

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Cover of 'Multipotent Stem Cells of the Hair Follicle'

Table of Contents

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    Book Overview
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    Chapter 1 Multipotent Stem Cells of the Hair Follicle
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    Chapter 2 Nestin-Based Reporter Transgenic Mouse Lines.
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    Chapter 3 Discovery of HAP Stem Cells.
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    Chapter 4 Multipotent Stem Cells of the Hair Follicle
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    Chapter 5 Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells.
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    Chapter 6 Nestin-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam® Histoculture.
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    Chapter 7 Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles.
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    Chapter 8 Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay.
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    Chapter 9 Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ.
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    Chapter 10 Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation.
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    Chapter 11 Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.
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    Chapter 12 Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination.
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    Chapter 13 Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase.
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    Chapter 14 Protocols for Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice.
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    Chapter 15 Protocols for Gelfoam(®) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods.
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    Chapter 16 Multipotent Stem Cells of the Hair Follicle
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    Chapter 17 Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium.
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    Chapter 18 Multipotent Stem Cells of the Hair Follicle
Attention for Chapter 11: Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.
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Chapter title
Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.
Chapter number 11
Book title
Multipotent Stem Cells of the Hair Follicle
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3786-8_11
Pubmed ID
Book ISBNs
978-1-4939-3784-4, 978-1-4939-3786-8
Authors

Eduardo Martínez-Martínez, Eileen Uribe-Querol, Claudio I. Galván-Hernández, Gabriel Gutiérrez-Ospina

Editors

Robert M Hoffman

Abstract

We describe a stereology method to obtain reliable estimates of the total number of proliferative and migratory epithelial cells after wounding. Using pulse and chase experiments with halogenated thymidine analogs such as iododeoxyuridine (IdU) and chlorodeoxyuridine (CldU), it is possible to track epithelial populations with heterogeneous proliferative characteristics through skin compartments. The stereological and tissue processing methods described here apply widely to address important questions of skin stem-cell biology.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 25%
Student > Doctoral Student 3 25%
Unspecified 1 8%
Student > Bachelor 1 8%
Student > Master 1 8%
Other 2 17%
Unknown 1 8%
Readers by discipline Count As %
Medicine and Dentistry 6 50%
Biochemistry, Genetics and Molecular Biology 1 8%
Environmental Science 1 8%
Agricultural and Biological Sciences 1 8%
Veterinary Science and Veterinary Medicine 1 8%
Other 1 8%
Unknown 1 8%