Protocols for Gelfoam(®) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods.
Multipotent Stem Cells of the Hair Follicle
Methods in molecular biology, January 2016
Wenluo Cao, Fang Liu, Yasuyuki Amoh, Robert M. Hoffman
Robert M Hoffman
Gelfoam(®)-histocultured whisker follicles from nestin-driven-green fluorescent protein (ND-GFP) mice produced growing pigmented and unpigmented hair shafts. Hair-shaft length increased rapidly by day 4 and continued growing until at least day 12 after which the hair-shaft length was constant. By day 63 in histoculture, the number of ND-GFP hair follicle-associated pluripotent (HAP) stem cells increased significantly and the follicles were intact. Three-dimensional Gelfoam(®) histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.
|Readers by professional status||Count||As %|
|Student > Doctoral Student||2||67%|
|Readers by discipline||Count||As %|
|Medicine and Dentistry||2||67%|