Title |
α‐synuclein assemblies sequester neuronal α3‐Na+/K+‐ATPase and impair Na+ gradient
|
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Published in |
EMBO Journal, August 2015
|
DOI | 10.15252/embj.201591397 |
Pubmed ID | |
Authors |
Amulya Nidhi Shrivastava, Virginie Redeker, Nicolas Fritz, Laura Pieri, Leandro G Almeida, Maria Spolidoro, Thomas Liebmann, Luc Bousset, Marianne Renner, Clément Léna, Anita Aperia, Ronald Melki, Antoine Triller |
Abstract |
Extracellular α-synuclein (α-syn) assemblies can be up-taken by neurons; however, their interaction with the plasma membrane and proteins has not been studied specifically. Here we demonstrate that α-syn assemblies form clusters within the plasma membrane of neurons. Using a proteomic-based approach, we identify the α3-subunit of Na(+)/K(+)-ATPase (NKA) as a cell surface partner of α-syn assemblies. The interaction strength depended on the state of α-syn, fibrils being the strongest, oligomers weak, and monomers none. Mutations within the neuron-specific α3-subunit are linked to rapid-onset dystonia Parkinsonism (RDP) and alternating hemiplegia of childhood (AHC). We show that freely diffusing α3-NKA are trapped within α-syn clusters resulting in α3-NKA redistribution and formation of larger nanoclusters. This creates regions within the plasma membrane with reduced local densities of α3-NKA, thereby decreasing the efficiency of Na(+) extrusion following stimulus. Thus, interactions of α3-NKA with extracellular α-syn assemblies reduce its pumping activity as its mutations in RDP/AHC. |
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United Kingdom | 2 | 50% |
United States | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
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France | 1 | <1% |
Canada | 1 | <1% |
Unknown | 162 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 37 | 22% |
Student > Ph. D. Student | 25 | 15% |
Student > Bachelor | 17 | 10% |
Student > Master | 17 | 10% |
Student > Doctoral Student | 9 | 5% |
Other | 23 | 14% |
Unknown | 38 | 23% |
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Neuroscience | 44 | 27% |
Agricultural and Biological Sciences | 30 | 18% |
Biochemistry, Genetics and Molecular Biology | 25 | 15% |
Medicine and Dentistry | 15 | 9% |
Chemistry | 4 | 2% |
Other | 10 | 6% |
Unknown | 38 | 23% |