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Autophagy‐linked plasma and lysosomal membrane protein PLAC8 is a key host factor for SARS‐CoV‐2 entry into human cells

Overview of attention for article published in EMBO Journal, October 2022
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#9 of 12,129)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
37 news outlets
blogs
1 blog
twitter
48 X users
facebook
1 Facebook page

Citations

dimensions_citation
16 Dimensions

Readers on

mendeley
27 Mendeley
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Title
Autophagy‐linked plasma and lysosomal membrane protein PLAC8 is a key host factor for SARS‐CoV‐2 entry into human cells
Published in
EMBO Journal, October 2022
DOI 10.15252/embj.2022110727
Pubmed ID
Authors

Alejandro P Ugalde, Gabriel Bretones, David Rodríguez, Víctor Quesada, Francisco Llorente, Raúl Fernández‐Delgado, Miguel Ángel Jiménez‐Clavero, Jesús Vázquez, Enrique Calvo, Isaac Tamargo‐Gómez, Guillermo Mariño, David Roiz‐Valle, Daniel Maeso, Miguel Araujo‐Voces, Yaiza Español, Carles Barceló, José MP Freije, Alejandro López‐Soto, Carlos López‐Otín

Abstract

Better understanding interactions between SARS-CoV-2 and host cells should help to identify host factors that may be targetable to combat infection and COVID-19 pathology. To this end, we have conducted a genome-wide CRISPR/Cas9-based loss-of-function screen in human lung cancer cells infected with SARS-CoV-2-pseudotyped lentiviruses. Our results recapitulate many findings from previous screens that used full SARS-CoV-2 viruses, but also unveil two novel critical host factors: the lysosomal efflux transporter SPNS1 and the plasma and lysosomal membrane protein PLAC8. Functional experiments with full SARS-CoV-2 viruses confirm that loss-of-function of these genes impairs viral entry. We find that PLAC8 is a key limiting host factor, whose overexpression boosts viral infection in eight different human lung cancer cell lines. Using single-cell RNA-Seq data analyses, we demonstrate that PLAC8 is highly expressed in ciliated and secretory cells of the respiratory tract as well as in gut enterocytes, cell types that are highly susceptible to SARS-CoV-2 infection. Proteomics and cell biology studies suggest that PLAC8 and SPNS1 regulate the autophagolysosomal compartment and affect the intracellular fate of endocytosed virions.

X Demographics

X Demographics

The data shown below were collected from the profiles of 48 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 19%
Researcher 3 11%
Other 2 7%
Student > Doctoral Student 2 7%
Student > Master 2 7%
Other 3 11%
Unknown 10 37%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 22%
Medicine and Dentistry 4 15%
Agricultural and Biological Sciences 2 7%
Unspecified 1 4%
Mathematics 1 4%
Other 3 11%
Unknown 10 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 314. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 January 2024.
All research outputs
#109,244
of 25,537,395 outputs
Outputs from EMBO Journal
#9
of 12,129 outputs
Outputs of similar age
#3,307
of 440,712 outputs
Outputs of similar age from EMBO Journal
#1
of 81 outputs
Altmetric has tracked 25,537,395 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,129 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.7. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 440,712 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 81 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.