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Ex vivo DNA Assembly

Overview of attention for article published in Frontiers in Bioengineering and Biotechnology, January 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (91st percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

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22 X users
facebook
1 Facebook page
googleplus
2 Google+ users
reddit
1 Redditor

Citations

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3 Dimensions

Readers on

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51 Mendeley
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Title
Ex vivo DNA Assembly
Published in
Frontiers in Bioengineering and Biotechnology, January 2013
DOI 10.3389/fbioe.2013.00012
Pubmed ID
Authors

Adam B. Fisher, Zachary B. Canfield, Laura C. Hayward, Stephen S. Fong, George H. McArthur

Abstract

Even with decreasing DNA synthesis costs there remains a need for inexpensive, rapid, and reliable methods for assembling synthetic DNA into larger constructs or combinatorial libraries. Advances in cloning techniques have resulted in powerful in vitro and in vivo assembly of DNA. However, monetary and time costs have limited these approaches. Here, we report an ex vivo DNA assembly method that uses cellular lysates derived from a commonly used laboratory strain of Escherichia coli for joining double-stranded DNA with short end homologies embedded within inexpensive primers. This method concurrently shortens the time and decreases costs associated with current DNA assembly methods.

X Demographics

X Demographics

The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Australia 1 2%
United Kingdom 1 2%
Canada 1 2%
Belgium 1 2%
Japan 1 2%
United States 1 2%
Unknown 44 86%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 22%
Student > Bachelor 11 22%
Student > Ph. D. Student 7 14%
Student > Master 6 12%
Student > Postgraduate 3 6%
Other 7 14%
Unknown 6 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 59%
Biochemistry, Genetics and Molecular Biology 11 22%
Engineering 2 4%
Immunology and Microbiology 1 2%
Medicine and Dentistry 1 2%
Other 1 2%
Unknown 5 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2023.
All research outputs
#2,583,275
of 25,758,695 outputs
Outputs from Frontiers in Bioengineering and Biotechnology
#317
of 8,648 outputs
Outputs of similar age
#24,448
of 291,042 outputs
Outputs of similar age from Frontiers in Bioengineering and Biotechnology
#3
of 19 outputs
Altmetric has tracked 25,758,695 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,648 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 291,042 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.