Title |
Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and Applications
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Published in |
Frontiers in Bioengineering and Biotechnology, September 2015
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DOI | 10.3389/fbioe.2015.00147 |
Pubmed ID | |
Authors |
Frank Delvigne, Hélène Pêcheux, Cédric Tarayre |
Abstract |
The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a particular promoter, not only the synthesis of a specific protein but also the content of intracellular metabolites. The level of protein/metabolite is thus proportional to a fluorescence signal. By this way, mean expression profiles of protein/metabolites can be determined non-invasively at a high-throughput rate, allowing the rapid identification of the best producers. Actually, different kinds of reporter systems are available, as well as specific cultivation devices allowing the on-line recording of the fluorescent signal. Cell-to-cell variability is another important phenomenon that can be integrated into the screening procedures for the selection of more efficient microbial cell factories. |
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Geographical breakdown
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France | 1 | 50% |
Switzerland | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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United States | 1 | 1% |
Belgium | 1 | 1% |
Unknown | 73 | 97% |
Demographic breakdown
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Student > Bachelor | 18 | 24% |
Student > Master | 10 | 13% |
Student > Ph. D. Student | 10 | 13% |
Researcher | 9 | 12% |
Student > Doctoral Student | 5 | 7% |
Other | 8 | 11% |
Unknown | 15 | 20% |
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Chemistry | 2 | 3% |
Other | 4 | 5% |
Unknown | 21 | 28% |