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Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors

Overview of attention for article published in Frontiers in Bioengineering and Biotechnology, November 2015
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Title
Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors
Published in
Frontiers in Bioengineering and Biotechnology, November 2015
DOI 10.3389/fbioe.2015.00189
Pubmed ID
Authors

Christina Dickmeis, Mareike Michaela Antonia Honickel, Rainer Fischer, Ulrich Commandeur

Abstract

We have generated hybrid chimeric potato virus X (PVX) particles by coexpression of different PVX coat protein fusions utilizing tobacco mosaic virus (TMV) and PVX-based expression vectors. Coinfection was achieved with a modified PVX overcoat vector displaying a fluorescent protein and a TMV vector expressing another PVX fluorescent overcoat fusion protein. Coexpression of the PVX-CP fusions in the same cells was confirmed by epifluorescence microscopy. Labeling with specific antibodies and transmission electron microscopy revealed chimeric particles displaying green fluorescent protein and mCherry on the surface. These data were corroborated by bimolecular fluorescence complementation. We used split-mCherry fragments as PVX coat fusions and confirmed an interaction between the split-mCherry fragments in coinfected cells. The presence of assembled split-mCherry on the surface confirmed the hybrid character of the chimeric particles.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 3%
United States 1 3%
Unknown 33 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 20%
Student > Master 6 17%
Other 5 14%
Student > Bachelor 5 14%
Student > Ph. D. Student 4 11%
Other 2 6%
Unknown 6 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 49%
Biochemistry, Genetics and Molecular Biology 8 23%
Business, Management and Accounting 1 3%
Computer Science 1 3%
Materials Science 1 3%
Other 0 0%
Unknown 7 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2015.
All research outputs
#18,430,915
of 22,833,393 outputs
Outputs from Frontiers in Bioengineering and Biotechnology
#3,398
of 6,565 outputs
Outputs of similar age
#278,564
of 386,526 outputs
Outputs of similar age from Frontiers in Bioengineering and Biotechnology
#40
of 62 outputs
Altmetric has tracked 22,833,393 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,565 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 386,526 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 62 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.