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Attention Score in Context
| Title |
Metabolic Engineering of Pseudomonas putida KT2440 for the Production of para-Hydroxy Benzoic Acid
|
|---|---|
| Published in |
Frontiers in Bioengineering and Biotechnology, November 2016
|
| DOI | 10.3389/fbioe.2016.00090 |
| Pubmed ID | |
| Authors |
Shiqin Yu, Manuel R. Plan, Gal Winter, Jens O. Krömer |
| Abstract |
para-Hydroxy benzoic acid (PHBA) is the key component for preparing parabens, a common preservatives in food, drugs, and personal care products, as well as high-performance bioplastics such as liquid crystal polymers. Pseudomonas putida KT2440 was engineered to produce PHBA from glucose via the shikimate pathway intermediate chorismate. To obtain the PHBA production strain, chorismate lyase UbiC from Escherichia coli and a feedback resistant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase encoded by gene aroG(D146N) were overexpressed individually and simultaneously. In addition, genes related to product degradation (pobA) or competing for the precursor chorismate (pheA and trpE) were deleted from the genome. To further improve PHBA production, the glucose metabolism repressor hexR was knocked out in order to increase erythrose 4-phosphate and NADPH supply. The best strain achieved a maximum titer of 1.73 g L(-1) and a carbon yield of 18.1% (C-mol C-mol(-1)) in a non-optimized fed-batch fermentation. This is to date the highest PHBA concentration produced by P. putida using a chorismate lyase. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 110 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 110 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 19% |
| Student > Master | 15 | 14% |
| Researcher | 15 | 14% |
| Student > Bachelor | 13 | 12% |
| Student > Doctoral Student | 5 | 5% |
| Other | 8 | 7% |
| Unknown | 33 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 31 | 28% |
| Agricultural and Biological Sciences | 23 | 21% |
| Chemical Engineering | 4 | 4% |
| Environmental Science | 4 | 4% |
| Unspecified | 2 | 2% |
| Other | 8 | 7% |
| Unknown | 38 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2016.
All research outputs
#18,483,671
of 22,903,988 outputs
Outputs from Frontiers in Bioengineering and Biotechnology
#3,414
of 6,655 outputs
Outputs of similar age
#304,687
of 416,651 outputs
Outputs of similar age from Frontiers in Bioengineering and Biotechnology
#12
of 20 outputs
Altmetric has tracked 22,903,988 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,655 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 416,651 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.