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Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli

Overview of attention for article published in Frontiers in Bioengineering and Biotechnology, December 2016
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Title
Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli
Published in
Frontiers in Bioengineering and Biotechnology, December 2016
DOI 10.3389/fbioe.2016.00096
Pubmed ID
Authors

Sonja J. Messerschmidt, Daniel Schindler, Celine M. Zumkeller, Franziska S. Kemter, Nadine Schallopp, Torsten Waldminghaus

Abstract

Learning by building is one of the core ideas of synthetic biology research. Consequently, building synthetic chromosomes is the way to fully understand chromosome characteristics. The last years have seen exciting synthetic chromosome studies. We had previously introduced the synthetic secondary chromosome synVicII in Escherichia coli. It is based on the replication mechanism of the secondary chromosome in Vibrio cholerae. Here, we present a detailed analysis of its genetic characteristics and a selection approach to optimize replicon stability. We probe the origin diversity of secondary chromosomes from Vibrionaceae by construction of several new respective replicons. Finally, we present a synVicII version 2.0 with several innovations including its full compatibility with the popular modular cloning (MoClo) assembly system.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 25%
Student > Bachelor 4 17%
Researcher 4 17%
Student > Ph. D. Student 3 13%
Student > Doctoral Student 3 13%
Other 2 8%
Unknown 2 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 50%
Agricultural and Biological Sciences 6 25%
Chemical Engineering 1 4%
Immunology and Microbiology 1 4%
Psychology 1 4%
Other 0 0%
Unknown 3 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 December 2016.
All research outputs
#18,498,050
of 22,919,505 outputs
Outputs from Frontiers in Bioengineering and Biotechnology
#3,420
of 6,663 outputs
Outputs of similar age
#309,680
of 420,056 outputs
Outputs of similar age from Frontiers in Bioengineering and Biotechnology
#10
of 17 outputs
Altmetric has tracked 22,919,505 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,663 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,056 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.