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Mendeley readers
Attention Score in Context
| Title |
Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo
|
|---|---|
| Published in |
Frontiers in Cell and Developmental Biology, July 2014
|
| DOI | 10.3389/fcell.2014.00030 |
| Pubmed ID | |
| Authors |
Yoshinori Makino, Erina Inoue, Masashi Hada, Keisuke Aoshima, Satsuki Kitano, Hitoshi Miyachi, Yuki Okada |
| Abstract |
In vivo fluorescent imaging technique is a strong tool to visualize the various cellular events such as the proliferation, differentiation, migration, and a lineage tracing in living cells requiring no further experimental procedure such as immunostaining. During spermatogenesis, unique and dynamic histone exchanges occur. Since the timing and types of histone exchanges defines the particular stages of spermatogenesis, visualizing certain types of histones in testes is useful not only for researching specific histone dynamics, but also for monitoring the stages of spermatogenesis in vivo. In this study, we report the establishment of two transgenic (Tg) mouse lines expressing histone H4-Venus (H4V) and histone H3.3-mCherry (H33C) fusion proteins in testicular germ cells, and demonstrated their utility for monitoring germ cell development in vivo. Because of the choice of promoter as well as the nature of these histones, H4V and H33C were exclusively expressed in the germ cells of the distinct stages, which allowed the determination of spermatogenic stages in real time. In addition, disappearance of H4V and H33C at particular stages of differentiation/fertilization also represented dynamic histone removal. Collectively, these Tg mice are a valuable resource not only for monitoring differentiation stages, but also for studying the chromatin dynamics of post-natal testicular germ cell development in vivo. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Colombia | 1 | 3% |
| Germany | 1 | 3% |
| Netherlands | 1 | 3% |
| Mexico | 1 | 3% |
| Japan | 1 | 3% |
| United States | 1 | 3% |
| Unknown | 31 | 84% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 24% |
| Student > Master | 7 | 19% |
| Student > Ph. D. Student | 7 | 19% |
| Student > Bachelor | 5 | 14% |
| Student > Doctoral Student | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 2 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 49% |
| Medicine and Dentistry | 6 | 16% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Unspecified | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 4 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2014.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from Frontiers in Cell and Developmental Biology
#5,727
of 10,472 outputs
Outputs of similar age
#175,255
of 239,854 outputs
Outputs of similar age from Frontiers in Cell and Developmental Biology
#16
of 24 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,472 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 239,854 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.