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Mendeley readers
Attention Score in Context
| Title |
Analysis of Antimicrobial-Triggered Membrane Depolarization Using Voltage Sensitive Dyes
|
|---|---|
| Published in |
Frontiers in Cell and Developmental Biology, April 2016
|
| DOI | 10.3389/fcell.2016.00029 |
| Pubmed ID | |
| Authors |
J. Derk te Winkel, Declan A. Gray, Kenneth H. Seistrup, Leendert W. Hamoen, Henrik Strahl |
| Abstract |
The bacterial cytoplasmic membrane is a major inhibitory target for antimicrobial compounds. Commonly, although not exclusively, these compounds unfold their antimicrobial activity by disrupting the essential barrier function of the cell membrane. As a consequence, membrane permeability assays are central for mode of action studies analysing membrane-targeting antimicrobial compounds. The most frequently used in vivo methods detect changes in membrane permeability by following internalization of normally membrane impermeable and relatively large fluorescent dyes. Unfortunately, these assays are not sensitive to changes in membrane ion permeability which are sufficient to inhibit and kill bacteria by membrane depolarization. In this manuscript, we provide experimental advice how membrane potential, and its changes triggered by membrane-targeting antimicrobials can be accurately assessed in vivo. Optimized protocols are provided for both qualitative and quantitative kinetic measurements of membrane potential. At last, single cell analyses using voltage-sensitive dyes in combination with fluorescence microscopy are introduced and discussed. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 50% |
| Switzerland | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 297 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 297 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 82 | 28% |
| Researcher | 46 | 15% |
| Student > Master | 30 | 10% |
| Student > Bachelor | 24 | 8% |
| Student > Doctoral Student | 18 | 6% |
| Other | 30 | 10% |
| Unknown | 67 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 80 | 27% |
| Agricultural and Biological Sciences | 49 | 16% |
| Immunology and Microbiology | 24 | 8% |
| Chemistry | 24 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 11 | 4% |
| Other | 31 | 10% |
| Unknown | 78 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2017.
All research outputs
#17,796,099
of 22,862,742 outputs
Outputs from Frontiers in Cell and Developmental Biology
#4,293
of 9,031 outputs
Outputs of similar age
#206,313
of 300,837 outputs
Outputs of similar age from Frontiers in Cell and Developmental Biology
#27
of 39 outputs
Altmetric has tracked 22,862,742 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,031 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,837 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.