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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis
|
|---|---|
| Published in |
Frontiers in Chemistry, June 2014
|
| DOI | 10.3389/fchem.2014.00034 |
| Pubmed ID | |
| Authors |
Seok Hoon Hong, Yong-Chan Kwon, Michael C. Jewett |
| Abstract |
Incorporating non-standard amino acids (NSAAs) into proteins enables new chemical properties, new structures, and new functions. In recent years, improvements in cell-free protein synthesis (CFPS) systems have opened the way to accurate and efficient incorporation of NSAAs into proteins. The driving force behind this development has been three-fold. First, a technical renaissance has enabled high-yielding (>1 g/L) and long-lasting (>10 h in batch operation) CFPS in systems derived from Escherichia coli. Second, the efficiency of orthogonal translation systems (OTSs) has improved. Third, the open nature of the CFPS platform has brought about an unprecedented level of control and freedom of design. Here, we review recent developments in CFPS platforms designed to precisely incorporate NSAAs. In the coming years, we anticipate that CFPS systems will impact efforts to elucidate structure/function relationships of proteins and to make biomaterials and sequence-defined biopolymers for medical and industrial applications. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Switzerland | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 244 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 2% |
| United Kingdom | 4 | 2% |
| Israel | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 233 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 73 | 30% |
| Researcher | 48 | 20% |
| Student > Bachelor | 38 | 16% |
| Student > Master | 28 | 11% |
| Student > Doctoral Student | 6 | 2% |
| Other | 20 | 8% |
| Unknown | 31 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 66 | 27% |
| Agricultural and Biological Sciences | 61 | 25% |
| Chemistry | 35 | 14% |
| Chemical Engineering | 13 | 5% |
| Engineering | 12 | 5% |
| Other | 22 | 9% |
| Unknown | 35 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2020.
All research outputs
#2,332,625
of 22,757,090 outputs
Outputs from Frontiers in Chemistry
#101
of 5,897 outputs
Outputs of similar age
#24,833
of 229,145 outputs
Outputs of similar age from Frontiers in Chemistry
#2
of 28 outputs
Altmetric has tracked 22,757,090 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,897 research outputs from this source. They receive a mean Attention Score of 2.0. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 229,145 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.