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1H high resolution magic-angle coil spinning (HR-MACS) μNMR metabolic profiling of whole Saccharomyces cervisiae cells: a demonstrative study

Overview of attention for article published in Frontiers in Chemistry, June 2014
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Title
1H high resolution magic-angle coil spinning (HR-MACS) μNMR metabolic profiling of whole Saccharomyces cervisiae cells: a demonstrative study
Published in
Frontiers in Chemistry, June 2014
DOI 10.3389/fchem.2014.00038
Pubmed ID
Authors

Alan Wong, Céline Boutin, Pedro M. Aguiar

Abstract

The low sensitivity and thus need for large sample volume is one of the major drawbacks of Nuclear Magnetic Resonance (NMR) spectroscopy. This is especially problematic for performing rich metabolic profiling of scarce samples such as whole cells or living organisms. This study evaluates a (1)H HR-MAS approach for metabolic profiling of small volumes (250 nl) of whole cells. We have applied an emerging micro-NMR technology, high-resolution magic-angle coil spinning (HR-MACS), to study whole Saccharomyces cervisiae cells. We find that high-resolution high-sensitivity spectra can be obtained with only 19 million cells and, as a demonstration of the metabolic profiling potential, we perform two independent metabolomics studies identifying the significant metabolites associated with osmotic stress and aging.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Switzerland 1 3%
Brazil 1 3%
Unknown 28 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 27%
Student > Ph. D. Student 5 17%
Student > Doctoral Student 3 10%
Student > Master 3 10%
Lecturer 2 7%
Other 4 13%
Unknown 5 17%
Readers by discipline Count As %
Chemistry 10 33%
Biochemistry, Genetics and Molecular Biology 7 23%
Agricultural and Biological Sciences 3 10%
Nursing and Health Professions 1 3%
Engineering 1 3%
Other 0 0%
Unknown 8 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2015.
All research outputs
#17,236,404
of 25,374,647 outputs
Outputs from Frontiers in Chemistry
#1,714
of 6,765 outputs
Outputs of similar age
#145,579
of 243,580 outputs
Outputs of similar age from Frontiers in Chemistry
#14
of 33 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 31st percentile – i.e., 31% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,765 research outputs from this source. They receive a mean Attention Score of 2.4. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 243,580 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.